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RNA Polymerase SP6, 20u/Ul (RNA) Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653900

Purified from an E.coli strain carrying a plasmid with the cloned gene encoding this enzyme. SP6 RNA Polymerase has a stringent specificity for its own promoter sequences and catalyzes the synthesis of RNA from ribonucleoside triphosphates in the presence of… More details

$495 / 2000 Units +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RNA Polymerase SP6, 20u/ul, Enzyme
Full Product Name
RNA Polymerase SP6, 20u/ul
Purity/Purification
Molecular Biology Grade
Preparation and Storage
-70 degree C
Storage Buffer
50mM Tris-HCl (pH 8.0), 150mM NaCl, 5mM DTT, 0.1mg/ml BSA, 0.5mM ELUGENT Detergent and 50% glycerol.
Unit Definition
One unit of enzyme incorporates 1 nanomole of AMP into a polynucleotide fraction (adsorbed on DE-81) in 60 minutes at 37 degree C.

Product Categories/Family for RNA Polymerase SP6, 20u/ul enzyme

Cloning; Cloning-Enzymes
Application Notes for RNA Polymerase SP6, 20u/ul enzyme
Generation of strand-specific RNA sequences (see Protocol for Synthesis of Strand-specific RNA Sequences and Protocol for Synthesis of High Specific Activity Radiolabeled RNA Sequences) that may be used:, Probes for hybridization (1) and genomic DNA sequencing (2), , RNase protection assays (3), , Antisense RNAs (4) , Templates for in vitro RNA translation analysis (5) , Substrates for studies of RNA splicing (6), RNA secondary structure and RNA-protein interactions (7)., Supplied with 20u/ul 0.25ml of 5X Transcription Buffer

Other Notes

Small volumes of RNA Polymerase SP6, 20u/ul enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RNA Polymerase SP6, 20u/ul purchase

MBS653900 is an enzyme targeting RNA Polymerase SP6, 20u/ul.
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