Phosphatase, Alkaline, Placental (ALPP) Enzyme

Name: ALPP Enzyme; Phosphatase, Alkaline, Placental; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS653906
Price: 985 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653906

MBS653906 is an enzyme targeting ALPP. Also known as Phosphatase, Alkaline, Placental, Human (PLAP, Alkaline Phosphatase Placental, Alkaline Phosphatase Placental Type, ALP, ALPP, Glycerophosphatase, PLAP-1, Regan Isozyme). Host: Human placenta. Native human… More details

$985 / 100 Units +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Phosphatase, Alkaline, Placental (ALPP), Enzyme

Full Product Name

Phosphatase, Alkaline, Placental, Human (PLAP, Alkaline Phosphatase Placental, Alkaline Phosphatase Placental Type, ALP, ALPP, Glycerophosphatase, PLAP-1, Regan Isozyme)

Product Gene Name

ALPP enzyme

Host

Human placenta

Form/Format

Supplied as liquid in PBS. No preservative added.

Purity/Purification

Highly Purified
~95% (Biuret method)

Preparation and Storage

Store undiluted at -20 degree C. Stable for 6 months at -20 degree C. Avoid freeze-thaw cycles. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

57,954 Da

Chromosome Location

Chromosome: 2; NC_000002.11 (233243348..233247599). Location: 2q37

OMIM

171800

3D Structure

ModBase 3D Structure for P05187

Activity

1,000u/ml
Specific Activity: 1,069u/mg solid (typical value; lot-specific)
Unit Defintion of Activity: One unit liberates one micromole of pnitrophenol per minute at 37 degree C, Tris buffer, pH 10.15

Product Categories/Family for ALPP enzyme

Molecular Biology; MB-Enzymes; Phosphatase

Applications Tested/Suitable for ALPP enzyme

ELISA (EL/EIA)

Application Notes for ALPP enzyme

Suitable for use in ELISA.

Other Notes

Small volumes of ALPP enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ALPP. It may not necessarily be applicable to this product.

NCBI GI #

178464

NCBI GeneID

250

NCBI Accession #

AAA51706.1

UniProt Primary Accession #

P05187

UniProt Secondary Accession #

P05188; P06861; Q53S78; Q96DB7

UniProt Related Accession #

P05187

NCBI Official Full Name

placental alkaline phosphatase

NCBI Official Synonym Full Names

alkaline phosphatase, placental

NCBI Official Symbol

ALPP

NCBI Official Synonym Symbols

ALP; PALP; PLAP; PLAP-1

UniProt Protein Name

Alkaline phosphatase, placental type

UniProt Synonym Protein Names

Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1

Protein Family

Alkaline phosphatase

UniProt Gene Name

ALPP

UniProt Synonym Gene Names

PLAP; PLAP-1

UniProt Entry Name

PPB1_HUMAN

NCBI Protein Information

alkaline phosphatase, placental type; Regan isozyme; glycerophosphatase; alkaline phosphomonoesterase; placental alkaline phosphatase 1; alkaline phosphatase Regan isozyme

NCBI Summary for ALPP

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]

UniProt Comments for ALPP

Catalytic activity: A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor: Binds 1 magnesium ion

By similarity.Binds 2 zinc ions

By similarity.

Subunit structure: Homodimer.

Subcellular location: Cell membrane; Lipid-anchor › GPI-anchor Ref.10 Ref.11.

Polymorphism: Placental ALP is highly polymorphic, there are at least three common alleles.

Miscellaneous: In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Sequence similarities: Belongs to the alkaline phosphatase family.

Sequence caution: The sequence AAA51708.1 differs from that shown. Reason: Erroneous initiation.

Research Articles on ALPP

1. Calculation of the electrostatic potentials within the active site of human placental alkaline phosphatase also suggests that the local positive electrostatic environment may account for its capability to distinguish various substrates

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ALPP enzyme

Reference Product	PubMed Publications
PTS enzyme	>1 publications with ALPP and PTS

Pathways associated with ALPP enzyme

Products by Pathway	Pathway Diagram
Folate Biosynthesis Pathway antibodies	Folate Biosynthesis Pathway Diagram
Folate Biosynthesis Pathway antibodies	Folate Biosynthesis Pathway Diagram

Diseases associated with ALPP enzyme

Disease Name	Pubmed Publications
Neoplasms Antibodies	>146 publications with ALPP and Neoplasms
Adenocarcinoma Antibodies	>26 publications with ALPP and Adenocarcinoma
Nervous System Diseases Antibodies	>19 publications with ALPP and Nervous System Diseases
Lung Diseases Antibodies	>11 publications with ALPP and Lung Diseases
Breast Neoplasms Antibodies	>6 publications with ALPP and Breast Neoplasms
Disorders of Sex Development Antibodies	>5 publications with ALPP and Disorders of Sex Development
Liver Diseases Antibodies	>4 publications with ALPP and Liver Diseases
Necrosis Antibodies	>3 publications with ALPP and Necrosis
Mental Disorders Antibodies	>2 publications with ALPP and Mental Disorders
Pain Antibodies	>2 publications with ALPP and Pain

Organs/Tissues associated with ALPP enzyme

Organ/Tissue Name	Pubmed Publications
Placenta Antibodies	>85 publications with ALPP and Placenta
Intestine Antibodies	>10 publications with ALPP and Intestine
Heart Antibodies	>7 publications with ALPP and Heart
Uterus Antibodies	>6 publications with ALPP and Uterus
Amniotic Fluid Antibodies	>1 publications with ALPP and Amniotic Fluid