Endoglycosidase H Enzyme

Name: Enzyme; Endoglycosidase H; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS653913
Price: 565 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653913

Suitable for studies of glycopeptide and glycoprotein with "High Mannose" sugar chains. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Endoglycosidase H, Enzyme

Full Product Name

Endoglycosidase H (Endo-b-N-acetylglucosaminidase H)

Host

Streptococcus griseus

Form/Format

Supplied as a lyophilized powder from 10mM Tris-HCl, pH7.2 Reconstitute with 0.1ml sterile, distilled, deionized water.
Appearance: Lyophilized powder

Purity/Purification

Molecular Biology Grade
Endoglycosidase H was purified from the culture fluid of Streptococcus griseus using DEAE-Sephadex and Sephadex G-75.

Preparation and Storage

-20 degree C
Stability: Stable at pH 5.0-9.0 at 37 degree C for 20 hours. Stable for 2 years at -20 degree C. Aliquots of dissolved enzymes may be stored at -20 degree C. Repeated freezing and thawing may decrease the enzyme acitivity.

Activity

0.1units
Protein: 6ug/vial
Specific Activity:
~30 units/mg protein

Contaminants

No detectable amount of various exoglycosidases or proteases

Quality Control

Endoglycosidase H (0.01 unit) was incubated with appropriate p-nitrophenylglycosides (0.1mg) in 0.2ml of citrate-phosphate buffer (pH 5.0) at 37 degree C for 12 hours. No activites of a- and b-galactosidase or a-L-fucosidase were observed. Absence of contaminating a-mannosidase was also confirmed by using oligosaccharides from ovalbumin and yeast mannan.
Endoglycosidase H (0.01 unit) was incubated with 10mg casein in 1ml of 0.1M Tris-CHl buffer at 37 degree C for 20 hours. 1ml of 10% TCA was added. Release of acid soluble material was below the limit of detection (OD275 less than 0.05).
To inhibit potential protease activity during extended Endo-H digestions, 1mM PMSF and/or 10mM pepstatin A may be added to incubations. PMSF is stable as a 2mM stock solution in absolute methanol.

Unit Definition

One unit is that amount of enzyme required to hydrolyze 1mmole of (Man)5-(GlcNAc)2Asn-[14C]-acetyl, at pH 5.0 and 37 degree C per minute at the saturated substrate concentration. Muramatsu's method is employed).

Product Categories/Family for Endoglycosidase H enzyme

Molecular Biology; MB-Enzymes

Other Notes

Small volumes of Endoglycosidase H enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Endoglycosidase H purchase

MBS653913 is an enzyme targeting Endoglycosidase H. Also known as Endoglycosidase H (Endo-b-N-acetylglucosaminidase H). Host: Streptococcus griseus.

Additional Details

NCBI/Uniprot data below describe general gene information for Endoglycosidase H. It may not necessarily be applicable to this product.

NCBI GI #

119107

NCBI Accession #

P04067.1

NCBI Official Full Name

Endo-beta-N-acetylglucosaminidase H

Protein Family

Endo-beta-N-acetylglucosaminidase

UniProt Comments for Endoglycosidase H

Cleaves asparagine-linked oligomannose and hybrid, but not complex, oligosaccharides from glycoproteins.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.