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Proteinase K Solution 20mg/Ml Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653959

Proteinase K is classified as a non-specific serine protease. It is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic orhydrophobic amino acids. More details

$190 / 1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Proteinase K Solution 20mg/ml, Enzyme
Full Product Name
Proteinase K Solution 20mg/ml
Form/Format
Supplied as a liquid in 10mM Tris HCl, pH7.5, 10mM calcium acetate, 40% glycerol.
Concentration
20mg/ml (lot specific)
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Grade
Molecular Biology Grade
Source
Tritirachium album limber
Nomenclature
EC 3.4.21.14
Solubility
Colorless, clear, complete
pH
7.5
Activity
>= 35.5 units/mg
Unit Definition
One unit liberates 1umole of Folin positive amino acids, measured as Tyrosine, at 37°C, pH 7.5, using urea-denatured hemoglobin as the substrate.
CAS No
39450-01-6
Advantages
- Ready to use, provided in liquid form containing 40% glycerol.
- Smaller contamination risk (liquid form).
- Can be stored at -20°C still having the enzyme in liquid form.
- Excellent stability.
Notes
- Proteinase K is not inactivated bymetal-chelating agents like EDTA, bythiol-reactive reagents (such asmonoiodoacetic acid,p-chloromercuribenzoate) or by specifictrypsin and chymotrypsin inhibitors. Phenylmethylsulfonyl fluoride and diisopropyl phosphorofluoridate completely inhibit the enzyme.
- Proteinase K is stable over a wide pHrange: 4-12.5.- Raising the temperature of the reactionfrom 37°C to 50°C-65°C can increase the activity of Proteinase K.
- Proteinase K can be inactivated at 95°C for 10 minutes (some enzymatic activity may still remain)
- Proteinase K retains its activity in thepresence of SDS (0.5-1%) or urea(1-4M).
- Depending on the application the enzyme is used in the absence orpresence of 0.2-1% SDS or 1-4M urea.

Product Categories/Family for Proteinase K Solution 20mg/ml enzyme

Molecular Biology; MB-Enzymes
Applications Tested/Suitable for Proteinase K Solution 20mg/ml enzyme
Isolation of high molecular weight DNA
Isolation of plasmid and genomic DNA
Isolation of RNA
Inactivation of RNase and DNase activities
Application Notes for Proteinase K Solution 20mg/ml enzyme
The recommended working concentration for Proteinase K is 0.05-1mg/ml. Use 3ul of a 20mg/ml solution per 1.5ml of bacterial culture. Optimal dilutions to be determined by researcher.

Other Notes

Small volumes of Proteinase K Solution 20mg/ml enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

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MBS653959 is an enzyme targeting Proteinase K Solution 20mg/ml.
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