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HNE (4-Hydroxy-2-nonenal), ELISA Control (BSA)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS656950

HNE is a major product of endogenous lipid peroxidation. The w-6-family (linoleic and arachidonic acids) of polyunsaturated fatty acids may produce HNE as a result of free radical attack. HNE is a highly reactive compound and it can react with several… More details

$940 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HNE, Biochemical
Full Product Name
HNE (4-Hydroxy-2-nonenal), ELISA Control (BSA)
Form/Format
Supplied as a liquid in 10mM Tris-buffer, pH 7.5, and 0.01% benzethonium chloride. Conjugated to BSA.
Purity/Purification
Purified
Purified
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note
BSA-HNE conjugate should only be used as a positive control to optimize the bloting conditions. It is not a representative of the protein bands that might be labeled with the anti-HNE in a given samples. Typically, complex proteins samples, such as liver or brain, will show many bands that are modified with HNE. There is no specific pattern that must be obtained with the antibodies.

Product Categories/Family for HNE biochemical

Applications Tested/Suitable for HNE biochemical
ELISA (EL/EIA), Western Blot (WB)
Application Notes for HNE biochemical
HNE-BSA conjugate can be used to coat ELISA plates (coat 1-10ug/ml) to test antibodies or used a known positive control for ELISA or Western (load 1-5ul/lane). BSA-HNE conjugate is ~65-70kD. Other aggregated bands may be visible as well.

Other Notes

Small volumes of HNE biochemical vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HNE purchase

MBS656950 is a biochemical targeting HNE. Also known as HNE (4-Hydroxy-2-nonenal), ELISA Control (BSA).
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