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Transferrin/ Transferrin - Transferrin Receptor Complex (TFRC) Antibody – Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS660050

Hybridoma clones for MAbs 2A2 and 1C10 have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of a Balb/c mice immunized with purified transferrin from pooled human plasma.Hybridoma clones for MAbs 8B9, 11D3 and 12A6. have been derived… More details

$485 / 1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Transferrin/ transferrin - transferrin receptor complex (TFRC), Monoclonal Antibody
Full Product Name
Monoclonal mouse anti-transferrin
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1 for MAbs 2A2, 1C10, 8B9, 11D3, 12A6
Form/Format
PBS, pH 7.4, 0.09% sodium azide (NaN3)
Purity/Purification
Chromatography on protein A Sepharose for MAbs 8B9, 11D3 and 12A6, and chromatography on protein G Sepharose for MAbs 2A2 and 1C10
Concentration
4.2 mg/mL (lot specific)
Specificity
Human transferrin for all MAbs
Complex between transferrin and soluble transferrin receptor for MAbs 8b9 and 11D3.

MAb 2A2 reacts with N-terminal domain of human transferrin. MABS 2A2 and 1C10 do not cross-react with mouse, rabbit, bovine transferrin and with human lactoferrin.
Preparation and Storage
+4°C (+2...+8°C allowed).
MAb
12A6
Material Safety Note
This product is for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropiate care must be taken when handling this product.
Applications Tested/Suitable for anti-TFRC antibody
ELISA (EIA)
Application Notes for anti-TFRC antibody
Detection of transferrin in serum by sandwich immunoassay. Detection of transferrin - transferrin receptor complex by sandwich ELISA. All MAbs are working in ELISA.

Recommended pairs for transferrin detection by sandwich immunoassay (capture - detection):
8B9 - 11D3
8B9 - 12A6
1C10 - 2A2
1C10 - 12A6
11D3 - 1C10
11D3 - 8B9

Recommended MAbs for transferrin-transferrin receptor complex detection by ELISA sandwich method using anti-transferrin receptor MAb 23D10 as coating:

23D10 - 8B9, 23D10 - 11D3
Mabs 2A2 and 1C10 inhibit proliferation of Con-A stimulated peripheral blood lymphocytes.

Other Notes

Small volumes of anti-TFRC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TFRC purchase

MBS660050 is an antibody targeting TFRC. Also known as Monoclonal mouse anti-transferrin.

Additional Details

NCBI/Uniprot data below describe general gene information for TFRC. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
transferrin receptor protein 1
NCBI Official Symbol
TFRC
UniProt Protein Name
Transferrin receptor protein 1
UniProt Synonym Protein Names
CD_antigen: CD71
UniProt Gene Name
TFRC
UniProt Synonym Gene Names
TR; TfR; TfR1; Trfr
UniProt Entry Name
TFR1_FELCA
NCBI Protein Information
transferrin receptor protein 1; TR; tfR; tfR1; trfr

UniProt Comments for TFRC

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (). Positively regulates T and B cell proliferation through iron uptake ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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