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NT-proBNP Antibody – Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS660177

MBS660177 is an antibody targeting NT-proBNP. Also known as Monoclonal mouse anti- human N-terminal proBNP (NT-proBNP). More details

$830 / 1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NT-proBNP, Monoclonal Antibody
Full Product Name
Monoclonal mouse anti- human N-terminal proBNP (NT-proBNP)
Product Gene Name
anti-NT-proBNP antibody
Clonality
Monoclonal
Isotype
IgG1 for MAbs 11D1, 5B6, 15D7, 7B5, 16E6, 18H5, 16F3
IgG2a for MAbs 29D12, 13G12, 24E11, 28F8
IgG2b for MAbs 15F11, 15C4
Clone Number
5B6, 15F11, 7B5, 13G12, 11D1, 16E6, 15D7, 15C4, 24E11, 28F8, 29D12, 18H5, 16F3
Form/Format
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Purity/Purification
Chromatography on protein A Sepharose
Specificity
Human NT-proBNP and proBNP.
Preparation and Storage
4 degree C
Clone Note
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic N-terminal pro brain natriuretic peptide (NT-proBNP), corresponding to amino acid residues 1-12 (MAbs 5B6, 29D12), a.a.r. 13-27 (MAbs 15F11, 13G12, 18H5, 7B5, 16F3), a.a.r. 28-45 (MAbs 11D1, 16E6), a.a.r. 46-60 (MAb 15D7) and a.a.r. 61-76 (MAbs 24E11, 28F8, 15C4). The peptides used for immunization were conjugated with carrier protein.
Application Notes for anti-NT-proBNP antibody
NT-proBNP and proBNP immunoassay. All MAbs are working in Western Blotting.
Best pairs for sandwich immunoassay (capture - detection): 15F11 - 24E11, 15C4 - 29D12, 15C4 - 13G12, 15C4 - 18H5. These pairs recognize the antigen in blood of patients with heart failure, unstable angina and mydegree Cardial infarction.
MAbs specific to peptides 5-12 (MAb 29D12) and 13-27 (MAbs 15F11, 13G12, 18H5, 7B5, 16F3) recognize circulating proBNP and can be used for the development of quantitative proBNP assays in pairs with anti-BNP MAbs. The best pairs (coating - conjugate):
50E1- 16F3
50E1- 18H5
7B5 - 2G9.

Product References and Citations for anti-NT-proBNP antibody

Search NT-proBNP references from PubMed.

Other Notes

Small volumes of anti-NT-proBNP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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