Anti-cTnI Monoclonal Antibody – Cat# MBS660324

Troponin I Cardiac (cTnI) Antibody – Monoclonal

Name: cTnI Antibody; Monoclonal; MyBioSource
Brand: MyBioSource
SKU: MBS660324
Price: 610 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS660324

MBS660324 is an antibody targeting cTnI. Also known as Monoclonal mouse anti-cardiac troponin I (cTnI), in vitro. More details

$610 / 1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Troponin I Cardiac (cTnI), Monoclonal Antibody

Full Product Name

Monoclonal mouse anti-cardiac troponin I (cTnI), in vitro

Product Gene Name

anti-cTnI antibody

Clonality

Monoclonal

Isotype

IgG1 for Y101, M18cc, 1017cc, 1039cc, M155cc, 560cc, 16A11cc, 16A12cc, 8E10cc, MF4cc, P4-14G5, 909, 810, 3C7, 228, 820, 247, 17F3, 84, M46, 625, 596, p45-10
IgG2a for 4C2cc, 10F4, 267
IgG2b for 19C7cc, C5
IgG3 for 916,

Clone Number

Y101, M18cc, 1017cc, 1039cc, 4C2cc, M155cc, 19C7cc, 560cc, 16A11cc, 16A12cc, 8E10cc, MF4cc

Form/Format

PBS, pH 7.4, 0.09 % sodium azide (NaN3)

Purity/Purification

Protein A chromatography

Specificity

None of the MAbs (except M46, 267, 596 and C5) have cross-reaction with skeletal muscle TnI. Cross-reactivity with skeletal TnI is less than 10% for M46, 267 and 596, and more than 50% for C5. 247 reacts only with free cTnI.

MAb: P4-14G5 Epitope (a.a.r.): 1 - 15
MAb: 916 Epitope (a.a.r.): 13 - 22
MAb: 909 Epitope (a.a.r.): 18 - 22
MAb: M18cc Epitope (a.a.r.): 18 - 28
MAb: 801 Epitope (a.a.r.): 18 - 35
MAb: 810 Epitope (a.a.r.): 22 - 31
MAb: 1017cc, 1039cc Epitope (a.a.r.): 22 - 40
MAb: 4C2cc Epitope (a.a.r.): 23 - 29
MAb: 3C7 Epitope (a.a.r.): 25 - 40
MAb: M155cc, 228, 820 Epitope (a.a.r.): 26 - 35
MAb: 10F4 Epitope (a.a.r.): 34 - 37
MAb: 19C7cc Epitope (a.a.r.): 41 - 49
MAb: 247 Epitope (a.a.r.): 65 - 74
MAb: 560cc Epitope (a.a.r.): 83 - 93
MAb: Y101 Epitope (a.a.r.): 83 - 100
MAb: 16A11cc, 16A12cc, 8E10ccEpitope (a.a.r.): 86 - 90
MAb: 17F3 Epitope (a.a.r.): 87 - 90
MAb: 84 Epitope (a.a.r.): 117 - 126
MAb: M46 Epitope (a.a.r.): 130 - 145
MAb: 625, 458, 596, 267 Epitope (a.a.r.): 169 - 178
MAb: C5 Epitope (a.a.r.): 186 - 192
MAb: MF4cc Epitope (a.a.r.): 190 - 196
MAb: p45-10 Epitope (a.a.r.): 195 – 209

Preparation and Storage

+4 degree C (+2 … +8 degree C allowed)

OMIM

phenotype 612422

Clone Number Note

Mouse monoclonal antibody produced in bioreactor. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.

Immunogen

Free human cTnI for 16A11cc, 16A12cc, 19C7cc, 4C2cc, 8E10cc, M155cc, M18cc, MF4cc, 10F4, 17F3, 458, 84, C5, M46
Native human cTn complex for 560cc, 228, 247, 625
Native human cTn complex and human cTnI peptides for 810, 820
Native human cTn complex and artificial human cTn complex for 801
Free human cTnI and native human cTn complex 267, 3C7, 596,
Free human cTnI and human cTnI peptides for 1017cc, 1039cc, 909, 916
Free human cTnI, native human cTn complex and human cTnI peptides for Y101
Human cTnI peptides for P4-14G5, p45-10

Material safety note

This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative.
Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Applications Tested/Suitable for anti-cTnI antibody

Immunoassay; Immunoprecipitation, Immunohistochemistry, Immunoaffinity purification.

Application Notes for anti-cTnI antibody

MAbs work in Western blotting. For better sensitivity in Western blotting we recommend using 19C7cc, 16A11cc and MF4cc.

Pair recommendations for quantitative cTnI sandwich immunoassays:

Assay type	Capture	Detection
1 + 1 1 + 1 1 + 1 1 + 1 1 + 1 1 + 1 1 + 1 2 + 1 2 + 2 2 + 2 2 + 2 2 + 2	19C7cc 19C7cc 625 560cc 4C2cc 625 625 19C7cc + MF4cc 916 + 560cc 801 + 560cc 909 + 560cc M18 + 560cc	16A11cc 560cc 19C7cc 458 19C7cc 1017cc 1039cc 7B9cc (specific to TnC, Cat.# MBS660323) 19C7cc + MF4cc 19C7cc + MF4cc 19C7cc + MF4cc 19C7cc + MF4cc

801, 810, 820, 909 and 916 work well as capture MAbs with 19C7cc as detection MAb.

Other Notes

Small volumes of anti-cTnI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for cTnI. It may not necessarily be applicable to this product.

NCBI GI #

48255883

NCBI GeneID

7139

NCBI Accession #

NP_001001432.1

UniProt Secondary Accession #

O60214; Q99596; Q99597; Q9BUF6; Q9UM96; A2TDB9; A8K3K6

UniProt Related Accession #

P45379

GenBank Nucleotide #

NM_001001432.2

NCBI Official Full Name

troponin T, cardiac muscle isoform 4

NCBI Official Synonym Full Names

troponin T type 2 (cardiac)

NCBI Official Symbol

TNNT2

NCBI Official Synonym Symbols

CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6

UniProt Protein Name

Troponin T, cardiac muscle

UniProt Synonym Protein Names

Cardiac muscle troponin T; cTnT

Protein Family

Troponin

UniProt Gene Name

TNNT2

UniProt Synonym Gene Names

TnTc; cTnT

UniProt Entry Name

TNNT2_HUMAN

NCBI Protein Information

troponin T, cardiac muscle; troponin T2, cardiac; cardiomyopathy, hypertrophic 2; cardiomyopathy, dilated 1D (autosomal dominant)

NCBI Summary for cTnI

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]

UniProt Comments for cTnI

TNNT2: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. Belongs to the troponin T family. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: sarcomere; troponin complex; cytosol; striated muscle thin filament

Molecular Function: troponin C binding; structural constituent of cytoskeleton; troponin I binding; ATPase activity; tropomyosin binding; actin binding

Biological Process: regulation of muscle contraction; metabolic process; atrial cardiac muscle morphogenesis; positive regulation of ATPase activity; ventricular cardiac muscle morphogenesis; sarcomere organization; response to calcium ion; negative regulation of ATPase activity; regulation of heart contraction; muscle filament sliding

Disease: Cardiomyopathy, Familial Restrictive, 3; Cardiomyopathy, Dilated, 1d; Cardiomyopathy, Familial Hypertrophic, 2

Research Articles on cTnI

1. abundance of TNNT1 AS2 correlated negatively with single muscle fiber-specific force after resistance training, while abundance of AS1 correlated negatively with V max.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-cTnI antibody

Reference Product	PubMed Publications
MYBPC3 antibody	>73 publications with cTnI and MYBPC3
TNNI3 antibody	>58 publications with cTnI and TNNI3
TPM1 antibody	>38 publications with cTnI and TPM1
MYL2 antibody	>24 publications with cTnI and MYL2
MYL3 antibody	>21 publications with cTnI and MYL3
TNNC1 antibody	>14 publications with cTnI and TNNC1
TTN antibody	>9 publications with cTnI and TTN
TNNI2 antibody	>1 publications with cTnI and TNNI2
TNNI1 antibody	>1 publications with cTnI and TNNI1

Pathways associated with anti-cTnI antibody

Products by Pathway	Pathway Diagram
Adrenergic Signaling In Cardiomyocytes Pathway antibodies	Adrenergic Signaling In Cardiomyocytes Pathway Diagram
Adrenergic Signaling In Cardiomyocytes Pathway antibodies	Adrenergic Signaling In Cardiomyocytes Pathway Diagram
Cardiac Progenitor Differentiation Pathway antibodies	Cardiac Progenitor Differentiation Pathway Diagram
Cardiac Muscle Contraction Pathway antibodies	Cardiac Muscle Contraction Pathway Diagram
Cardiac Muscle Contraction Pathway antibodies	Cardiac Muscle Contraction Pathway Diagram
Dilated Cardiomyopathy Pathway antibodies	Dilated Cardiomyopathy Pathway Diagram
Dilated Cardiomyopathy Pathway antibodies	Dilated Cardiomyopathy Pathway Diagram
Hypertrophic Cardiomyopathy (HCM) Pathway antibodies	Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
Hypertrophic Cardiomyopathy (HCM) Pathway antibodies	Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
Muscle Contraction Pathway antibodies	Muscle Contraction Pathway Diagram

Diseases associated with anti-cTnI antibody

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>162 publications with cTnI and Cardiovascular Diseases
Heart Diseases Antibodies	>158 publications with cTnI and Heart Diseases
Cardiomyopathies Antibodies	>120 publications with cTnI and Cardiomyopathies
Cardiomyopathy, Hypertrophic Antibodies	>102 publications with cTnI and Cardiomyopathy, Hypertrophic
Cardiomyopathy, Hypertrophic, Familial Antibodies	>49 publications with cTnI and Cardiomyopathy, Hypertrophic, Familial
Cardiomyopathy, Dilated Antibodies	>46 publications with cTnI and Cardiomyopathy, Dilated
Cardiomegaly Antibodies	>41 publications with cTnI and Cardiomegaly
Hypertrophy Antibodies	>38 publications with cTnI and Hypertrophy
Heart Failure Antibodies	>36 publications with cTnI and Heart Failure
Death, Sudden, Cardiac Antibodies	>34 publications with cTnI and Death, Sudden, Cardiac

Organs/Tissues associated with anti-cTnI antibody

Organ/Tissue Name	Pubmed Publications
Heart Antibodies	>124 publications with cTnI and Heart
Muscle Antibodies	>100 publications with cTnI and Muscle
Blood Antibodies	>54 publications with cTnI and Blood
Embryonic Tissue Antibodies	>16 publications with cTnI and Embryonic Tissue
Kidney Antibodies	>8 publications with cTnI and Kidney
Brain Antibodies	>6 publications with cTnI and Brain
Lung Antibodies	>5 publications with cTnI and Lung
Liver Antibodies	>3 publications with cTnI and Liver
Connective Tissue Antibodies	>2 publications with cTnI and Connective Tissue
Testis Antibodies	>2 publications with cTnI and Testis