Phosphorylated Cardiac Troponin I (cTnI) Protein

Research for reliable biomarkers of MI has identified increased cardiac troponin I CTNI phosphorylation in serum correlates with cardiac muscle injury. This protein is also an important constituent of the troponin complex critical for human cardiac and skeletal muscle relaxation following force development contraction. The 4 phosphorylated activated protein forms are generated by the kinase PKA and have differential Ca2 sensitivity. Using NBCI Blast database and genome resources to verify protein purity and sequences, cTNI has been isolated and purified from human heart and fully phosphoryated, including Ser 150, in vitro by a protein kinase A PKA dependent, AMPK activated mechanism. The conserved domain was further analyzed with basic local alignment tool and compared to other Ca2 dependent AMP activated proteins. Shipped freeze dried in multiple available sizes, this protein may be used in IHC, ELISA or biol chem assays as suggested by Pubmed publications listed in datasheet.

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Small volumes of cTnI protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.