Neuron-Specific Enolase (NSE) Active Protein

Name: NSE Active Protein; Neuron-Specific Enolase; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS664045
Price: 855 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS664045

MBS664045 is an active protein targeting NSE. Also known as Neuron-specific enolase (NSE). More details

$855 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Neuron-specific Enolase (NSE), Active Protein

Full Product Name

Neuron-specific enolase (NSE)

Product Gene Name

NSE active protein

Form/Format

Solution in 0.1 M Tris-HCl, 5 mM Mg2SO4, 0.25 M KCl, pH 5.1 adjusted with sodium phosphate.

Purity/Purification

Ion-exchange chromatography and gel-filtration.

Preparation and Storage

At -20 degree C. Avoid repeated freezing and thawing.

Molecular Weight

47,141 Da

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Source Note

Post mortal human brain.
Blood sample from the tissue donor was tested and found negative for HBsAg, HCV, HIV-1 and 2 antibodies, and syphilis.

Analysis

Purity > 95 % (SDS-PAGE).

Activity

Specific activity is determined for each lot at 25 degree C in 45 mM imidazole, pH 6.8, 45 mM KCl, 1.33 mM Mg2SO4; 1 mM 2-PG. One unit is defined as the amount of NSE converted 1 mol of 2-phospho-D-glycerate per min at 25 degree C (J.Bidegree Chem. 87, 1587-1594, 1980). Subunit composition is gamma-gamma.

Dry Ice Shipment

Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Applications Tested/Suitable for NSE active protein

ELISA (EIA)

Application Notes for NSE active protein

Tested in ELISA and immunoblotting with anti-NSE antibodies.

Other Notes

Small volumes of NSE active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NSE. It may not necessarily be applicable to this product.

NCBI GI #

2465396

NCBI GeneID

24334

NCBI Accession #

AAB72088.1

UniProt Related Accession #

P07323

NCBI Official Full Name

neuron-specific enolase

NCBI Official Synonym Full Names

enolase 2, gamma, neuronal

NCBI Official Symbol

Eno2

NCBI Official Synonym Symbols

NSE; RNEN3

UniProt Protein Name

Gamma-enolase

UniProt Synonym Protein Names

2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE

Protein Family

Gamma-enolase

UniProt Gene Name

Eno2

UniProt Synonym Gene Names

Eno-2; NSE

UniProt Entry Name

ENOG_RAT

NCBI Protein Information

gamma-enolase; neural enolase; neuron-specific enolase; 2-phospho-D-glycerate hydro-lyase

NCBI Summary for NSE

catalyzes the interconversion of 2-phosphoglycerate to phosphoenolpyruvate; may play a role in glycolysis [RGD, Feb 2006]

UniProt Comments for NSE

ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase; EC 4.2.1.11

Cellular Component: extracellular space; neuron projection; photoreceptor inner segment; phosphopyruvate hydratase complex; cell soma; cytoplasm; perikaryon; intracellular; myelin sheath

Molecular Function: protein homodimerization activity; protein heterodimerization activity; magnesium ion binding; phosphopyruvate hydratase activity

Biological Process: response to drug; glycolysis; response to organic cyclic substance; response to estradiol stimulus; gluconeogenesis

Research Articles on NSE

1. Hyperglycemia may impair mitochondrial function and alter glycolysis in glomerular epithelial cells by down-regulating the expression of ATP synthase beta subunit and enolase 2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NSE active protein

Reference Product	PubMed Publications
Aldoc active protein	>3 publications with NSE and Aldoc
Pck1 active protein	>3 publications with NSE and Pck1
Gpi active protein	>2 publications with NSE and Gpi
Pkm2 active protein	>2 publications with NSE and Pkm2
Aldoa active protein	>1 publications with NSE and Aldoa
Pklr active protein	>1 publications with NSE and Pklr

Pathways associated with NSE active protein

Products by Pathway	Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Gluconeogenesis Pathway antibodies	Gluconeogenesis Pathway Diagram
Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies	Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies	Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
Glucose Metabolism Pathway antibodies	Glucose Metabolism Pathway Diagram
Glycogen Storage Diseases Pathway antibodies	Glycogen Storage Diseases Pathway Diagram

Diseases associated with NSE active protein

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>8 publications with NSE and Disease Models, Animal
Nervous System Diseases Antibodies	>7 publications with NSE and Nervous System Diseases
Brain Diseases Antibodies	>5 publications with NSE and Brain Diseases
Adenocarcinoma Antibodies	>4 publications with NSE and Adenocarcinoma
Kidney Diseases Antibodies	>3 publications with NSE and Kidney Diseases
Inflammation Antibodies	>2 publications with NSE and Inflammation
Prostatic Neoplasms Antibodies	>2 publications with NSE and Prostatic Neoplasms
Hypertension Antibodies	>2 publications with NSE and Hypertension
Lung Diseases Antibodies	>2 publications with NSE and Lung Diseases
Neoplasms, Experimental Antibodies	>2 publications with NSE and Neoplasms, Experimental

Organs/Tissues associated with NSE active protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>17 publications with NSE and Brain
Embryonic Tissue Antibodies	>3 publications with NSE and Embryonic Tissue
Nerve Antibodies	>3 publications with NSE and Nerve
Heart Antibodies	>2 publications with NSE and Heart
Prostate Antibodies	>2 publications with NSE and Prostate
Eye Antibodies	>2 publications with NSE and Eye
Ovary Antibodies	>1 publications with NSE and Ovary
Connective Tissue Antibodies	>1 publications with NSE and Connective Tissue