CD51 Antibody – Mouse Monoclonal

Monoclonal

IgG1k

P2W7

Mouse

Human

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl

Purified Preservative Free

1.0 mg/ml (lot specific)

Store at 2 - 5 degree C. Open under aseptic conditions. Freeze/Thawing is not recommended.
Product should retain activity for at least 6 months after shipping date when stored as recommended.

Five x 105 cultured UM-SCC (squamous cell carcinoma) per tube were harvested by trypsinization, washed and pre incubated 5 minutes with 20ul of 250ug/ml human IgG (to block nonspecific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-CD51 antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.32 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.

Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2 um filtered and vialed under aseptic conditions.

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.

Human ocular melanoma cell line (V+B2)