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IgG3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666028

Information: Immunoglobulin expressed by the mouse plasmacytoma hybridoma SIPC 3385 is an IgG3? isotype of unknown specificity. It is used as a negative control when working with human cells. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IgG3, Monoclonal Antibody
Full Product Name
Mouse IgG3-Biotin
Product Gene Name
anti-IgG3 antibody
Product Synonym Names
Monoclonal Mouse IgG3/Biotin
Clonality
Monoclonal
Isotype
IgG3k
Clone Number
SIPC3385
Host
Mouse
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
Store at 2 - 5 degree C. Freeze/thawing not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Cells Tested
Human PBL, HPB-MLT, Nalm-6, and HEL (cells expressing Fc receptors were pre blocked with human IgG).
Performance
Mouse IgG3/Biotin was tested as a negative control in Flow cytometry at 5 ug/ml using Streptavidin R-phycoerythrin as a 2 degree reagent. All cells tested failed to stain or display a significant mean shift above a buffer background.
Conjugation
Biotin
Production
Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Buffer
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Applications Tested/Suitable for anti-IgG3 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)

Other Notes

Small volumes of anti-IgG3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IgG3 purchase

MBS666028 is an antibody targeting IgG3. Also known as Mouse IgG3-Biotin. Host: Mouse.
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