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CD70 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666063

Information: Human CD70 is not present on resting lymphocytes. CD70 is expressed after activation on about 20% of T cells and on about 70% of B cells. CD70 is a ligand for CD27 and it is also a member of the tumor necrosis factor gene family. Antibody BU69… More details

$530 / 120 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD70, Monoclonal Antibody
Full Product Name
anti-human CD70-R-PE
Product Gene Name
Product Synonym Names
Monoclonal anti-human CD70/R-PE
Clonality
Monoclonal
Isotype
IgG1
Clone Number
BU69
Host
Mouse
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Preparation and Storage
Store at 2-5°C.
Do not freeze! Protect from light.
Product should retain activity for at least after shipping date when stored as recommended.
OMIM
602840
Performance
Five x 105 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD70/R-PE at a 1:50 dilution (10 ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.33 log10 fluorescent units when compared to a Mouse IgG1/PE negative control (MBS666704) at a similar concentration. Binding was blocked when pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD70 antibody (MBS666352)
Production
Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody aPE were removed using size exclusion chromatography. Antibody conjugate is at 0.5 mg/ml with PE: Ab molar ratio of 1.21.
Immunogen
Human WM-1 (Waldenström’s macroglobulinemia) cell line
Applications Tested/Suitable for anti-CD70 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Application Notes for anti-CD70 antibody
1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)

Testing Data of anti-CD70 antibody

Mouse Anti-CD70 Monoclonal Antibody – Cat# MBS666063

Testing Data of anti-CD70 antibody

Binding of anti-CD70/PE to human Raji cells
Mouse Anti-CD70 Monoclonal Antibody, Testing Data – Cat# MBS666063

Product References and Citations for anti-CD70 antibody

R.Q. Hintzen, et al, (1994) J Immunol 152: 1762-1773. M.R. Bowman, et al, (1994) J Immunol 152: 1756-1761. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1137-1138. K. Agematsu, et al, (1995) J Immunol 154: 3627-3635.

Other Notes

Small volumes of anti-CD70 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD70 purchase

MBS666063 is an antibody targeting CD70. Also known as anti-human CD70-R-PE. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD70. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
CD70 antigen
NCBI Official Synonym Full Names
CD70 molecule
NCBI Official Symbol
CD70
NCBI Official Synonym Symbols
CD27L; CD27LG; TNFSF7
UniProt Protein Name
CD70 antigen
UniProt Synonym Protein Names
CD27 ligand; CD27-L; Tumor necrosis factor ligand superfamily member 7; CD_antigen: CD70
Protein Family
UniProt Gene Name
CD70
UniProt Synonym Gene Names
CD27L; CD27LG; TNFSF7; CD27-L
UniProt Entry Name
CD70_HUMAN
NCBI Protein Information
CD70 antigen; CD27-L; CD27 ligand; Ki-24 antigen; surface antigen CD70; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor (ligand) superfamily, member 7

NCBI Summary for CD70

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD70

CD70: Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. Belongs to the tumor necrosis factor family.

Protein type: Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; protease binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: cell proliferation; cell-cell signaling; immune response; signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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