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CD3 Antibody – Hamster Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666074

Information: Antibody 145-2C11binds to an extracellular epitope of the 25 kd CD3e molecule present on murine T cells (1). CD3 molecules associate with T cell Receptors and provide intracellular signaling mechanism. Cross linking Cell surface CD3 with soluble… More details

$605 / 120 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD3, Monoclonal Antibody
Full Product Name
anti-mouse CD3-APC
Product Gene Name
Product Synonym Names
Monoclonal anti-mouse CD3/APC
Clonality
Monoclonal
Isotype
IgG
Clone Number
145-2C11
Host
Hamster
Species Reactivity
Mouse
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA.
Concentration
Volume: 0.2ml (lot specific)
Preparation and Storage
Store at 2 - 5 degree C. Do not freeze! Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Molecular Weight
18,897 Da
3D Structure
ModBase 3D Structure for P79951
Performance
Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 ul of 300 ug/ml Mouse IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-Mouse CD3/APC at a 1:50 dilution (5 ug/ml). They were then washed three times, fixed and analyzed by FACS. A 44.4% sub population of the cells stained positive with a mean shift of 1.35 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-mouse CD3 antibody.
Conjugation
APC
Production
Protein G purified antibody from tissue culture supernatant was conjugated to Allophycocycanin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 250 ug/ml with an A650/A280 ratio of 2.26.
Buffer
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative)
Immunogen
Mouse BM10-37 T cell membrane prep
Applications Tested/Suitable for anti-CD3 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB), ELISA (EIA)
Application Notes for anti-CD3 antibody
1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)

Testing Data of anti-CD3 antibody

Hamster Anti-CD3 Monoclonal Antibody – Cat# MBS666074

Product References and Citations for anti-CD3 antibody

1) O Leo, J A Bluestone, et al. (1987) PNAS USA 84(5): 1374-1378. 2) L E Samelson, J A Bluestone, et al. (1987) J Immunol 139(8): 2708-2714.

Other Notes

Small volumes of anti-CD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD3 purchase

MBS666074 is an antibody targeting CD3. Also known as anti-mouse CD3-APC. Reactive in Mouse. Host: Hamster.

Additional Details

NCBI/Uniprot data below describe general gene information for CD3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
NCBI Official Full Name
cd3
UniProt Protein Name
CD3gamma/delta
UniProt Synonym Protein Names
CD3gamma/delta
Protein Family
UniProt Gene Name
cd3g
UniProt Entry Name
P79951_XENLA

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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