CD94 Antibody – Mouse Monoclonal

Monoclonal

IgG1

HP-3D9

Mouse

Human

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.

1.0 mg/ml (lot specific)

Store at 2 - 5 degree C. Freeze/thawing not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.

17,109 Da

602894

ModBase 3D Structure for Q13241

Ficoll prepared human peripheral blood mononuclear cells were stimulated by incubating 1 day at 2 x 106 cells/ml in RPMI 10% FBS media including 5 mug/ml Phytohemagglutinin-P. Five x 105 cells per tube were then washed and pre incubated 10 minutes with 20 mul human IgG at 250 mug/ml (to block non specific binding) after which they were incubated 45 minutes on ice with 80 mul of anti-CD94/Biotin at 5 mug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. A net 20% sub population of the cells stained positive with a mean shift of 1.25 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 mul of 0.5 mg/ml anti-CD94 antibody.

Biotin

Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).

Cultured human NK cells