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IgG2a Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666101

Information: Immunoglobulin expressed by the mouse plasmacytoma hybridoma RPC 5 is an IgG2a isotype of unknown specificity. It is used as a negative control when working with human cells. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IgG2a, Monoclonal Antibody
Full Product Name
Mouse IgG2a-FITC
Product Gene Name
anti-IgG2a antibody
Product Synonym Names
Monoclonal Mouse IgG2a/FITC
Clonality
Monoclonal
Isotype
IgG2ak
Clone Number
RPC5
Host
Mouse
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Concentration
0.5 mg/ml (lot specific)
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Performance
Mouse IgG2a/FITC was tested as a negative control in Flow cytometry at 5 ug/ml. All cells tested failed to stain or display a significant mean shift above a buffer background. Cells tested: Human PBL, Jurkat, HPB-MLT, Nalm-6, Raji, CEM (Cells were pre incubated with human IgG to block nonspecific binding).
Conjugation
FITC
Production
Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to 95% Immunoglobulin by SDS-PAGE, and reacted with FITC. Unconjugated FITC was removed from conjugate using a desalting column. Antibody conjugate is at 0.5 mg/ml.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Applications Tested/Suitable for anti-IgG2a antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), ELISA (EIA)

Other Notes

Small volumes of anti-IgG2a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IgG2a purchase

MBS666101 is an antibody targeting IgG2a. Also known as Mouse IgG2a-FITC. Host: Mouse.
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