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CD137L Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666114

Information: Human CD137 Ligand is a type II transmembrane protein constitutively expressed by monocytes, B cells neuroblastoma cells (1), and some activated T cells. The interaction of CD137 (4-1BB) and CD137L can supply a costimulatory signal to T cells… More details

$605 / 120 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD137L, Monoclonal Antibody
Full Product Name
anti-human CD137L-R-PE
Product Gene Name
anti-CD137L antibody
Product Synonym Names
Monoclonal anti-human CD137L (4-1BB Ligand)/R-PE
Clonality
Monoclonal
Isotype
IgG2ak
Clone Number
ANC5D6
Host
Mouse
Species Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA.
Concentration
Volume: 0.2 ml (lot specific)
Preparation and Storage
Store at 2 - 5 degree C. Do not freeze! Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Performance
Five x 105 cultured human Raji cells per tube were washed and incubated 45 minutes on ice with 80 ul of anti-CD137L/R-PE at a 1:50 dilution factor (10 ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.54 log10 fluorescent units when compared to a Mouse IgG2a/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD137L antibody.
Conjugation
PE
Production
Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.5 mg/ml with an A565/A280 ratio of 2.9.
Buffer
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen
Recombinant human CD137L
Applications Tested/Suitable for anti-CD137L antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
Application Notes for anti-CD137L antibody
1:50 (or use 1.6ul of concentrated reagent per 5x105-cell test)

Testing Data of anti-CD137L antibody

Mouse Anti-CD137L Monoclonal Antibody – Cat# MBS666114

Product References and Citations for anti-CD137L antibody

1) M.R. Alderson, et al, (1994) Eur J Immunol 24: 2219-2227. 2) J. Langstein, et al, (1998) J Immunol 160: 2488-2494. 3) V.Y. Taraban, et al, (2002) Eur J. Immunol. 32: 3617-3627. 4) H. Schwartz, et al, (1996) Blood 87: 2839. 5) G. Zhu, et al, (2001) J Immunol 167: 2671-6.

Other Notes

Small volumes of anti-CD137L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD137L purchase

MBS666114 is an antibody targeting CD137L. Also known as anti-human CD137L-R-PE. Reactive in Human. Host: Mouse.
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