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CD13 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666116

Information: Human CD13 is a zinc-binding aminopeptidase-N enzyme expressed on the surface of myeloid cells. IL-4 will upregulate expression of CD13 which may play an anti-inflammatory role. Antibody 22A5 recognizes the cell surface aminopeptidase-N enzyme. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD13, Monoclonal Antibody
Full Product Name
anti-human CD13-Purified Preservative Free
Product Gene Name
anti-CD13 antibody
Product Synonym Names
Monoclonal anti-human CD13
Clonality
Monoclonal
Isotype
IgG2ak
Clone Number
22A5
Host
Mouse
Species Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
Purity/Purification
Purified Preservative Free
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
Store at 2 - 5 degree C. Open under aseptic conditions. Freeze/Thawing is not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended
Performance
Five x 105 cultured THP-1 cells were washed and pre incubated 5 minutes with 20ul of 250ug/ml human IgG (to block nonspecific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD13 antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.53 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration.
Production
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2 um filtered and vialed under aseptic conditions.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
Immunogen
Human osteosarcoma tissue
Applications Tested/Suitable for anti-CD13 antibody
Flow Cytometry (FC/FACS), FPS

Testing Data of anti-CD13 antibody

Mouse Anti-CD13 Monoclonal Antibody – Cat# MBS666116

Product References and Citations for anti-CD13 antibody

M.A. Horton, et al, (1985) Cancer Res 45: 5663-5669. R.A. Ashmun & A.T. Look (1990) Blood 75: 462-471. P.T.W. van Hal, et al, (1994) J Immunol 153: 2718-2728

Other Notes

Small volumes of anti-CD13 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD13 purchase

MBS666116 is an antibody targeting CD13. Also known as anti-human CD13-Purified Preservative Free. Reactive in Human. Host: Mouse.
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