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CD48 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666122

Information: Human CD48 is a GPI anchored cell surface gylcoprotein found on all lymphocytes and monocytes. CD48 is a ligand for CD2 and may be involved in enhancing the activating signal to B cells. Antibody 5-4.8 recognizes epitope 4 of the CD48 molecule of… More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD48, Monoclonal Antibody
Full Product Name
anti-human CD48-Purified Preservative Free
Product Gene Name
Product Synonym Names
Monoclonal anti-human CD48(Blast-1)
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
5-4.8
Host
Mouse
Species Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
Purity/Purification
Purified Preservative Free
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
Store at 2 - 5 degree C. Open under aseptic conditions. Freeze/Thawing is not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Molecular Weight
19,429 Da
OMIM
109530
Performance
Five x 105 cultured human Raji cells per tube were washed and incubated 45 minutes on ice with 80 ul of anti-CD48 antibody at a concentration of 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.87 log10 fluorescent units when compared to a Mouse IgG2a/FITC negative control at a similar concentration.
Production
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2 um filtered and vialed under aseptic conditions.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
Immunogen
Human PBLs
Applications Tested/Suitable for anti-CD48 antibody
Flow Cytometry (FC/FACS)

Testing Data of anti-CD48 antibody

Mouse Anti-CD48 Monoclonal Antibody – Cat# MBS666122

Product References and Citations for anti-CD48 antibody

(1) H.A. Vaughan, et al, (1983) Transplantation 36: 446-450. (2) H.A. Vaughan, et al, (1991) Immunogenetics 33: 113-117. (3)E.N. Klyushnenkova, et al, (1996) Cellular Immunol 174: 90-98. (4)Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 509-512.

Other Notes

Small volumes of anti-CD48 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD48 purchase

MBS666122 is an antibody targeting CD48. Also known as anti-human CD48-Purified Preservative Free. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD48. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
CD48 antigen isoform 2
NCBI Official Synonym Full Names
CD48 molecule
NCBI Official Symbol
CD48
NCBI Official Synonym Symbols
BCM1; BLAST; hCD48; mCD48; BLAST1; SLAMF2; MEM-102
UniProt Protein Name
CD48 antigen
UniProt Synonym Protein Names
B-lymphocyte activation marker BLAST-1; BCM1 surface antigen; Leukocyte antigen MEM-102; SLAM family member 2; SLAMF2; Signaling lymphocytic activation molecule 2; TCT.1; CD_antigen: CD48
Protein Family
UniProt Gene Name
CD48
UniProt Synonym Gene Names
BCM1; BLAST1; SLAMF2
UniProt Entry Name
CD48_HUMAN
NCBI Protein Information
CD48 antigen; TCT.1; BCM1 surface antigen; SLAM family member 2; leukocyte antigen MEM-102; B-lymphocyte activation marker BLAST-1; CD48 antigen (B-cell membrane protein); signaling lymphocytic activation molecule 2

NCBI Summary for CD48

This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The encoded protein is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
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UniProt Comments for CD48

CD48: Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 1q21.3-q22

Cellular Component: membrane; integral to plasma membrane; plasma membrane; lipid raft

Molecular Function: protein binding; receptor activity

Biological Process: defense response; blood coagulation; leukocyte migration
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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