• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CD25 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666125

Information: Expression of human CD25 (the alpha subunit of the IL-2 receptor complex) is dramatically upregulated on activated T cells, B cells, NK cells and macrophages (3). Antibodies to CD25 are being studied for potential use as an immundirected therapy… More details

$605 / 120 Tests +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD25, Monoclonal Antibody
Full Product Name
anti-human CD25-APC
Product Gene Name
Product Synonym Names
Monoclonal anti-human CD25(IL2-R alpha, TAC)/APC
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
7G7B6
Host
Mouse
Species Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA.
Concentration
Volume: 0.2 ml (lot specific)
Preparation and Storage
Store at 2 - 5 degree C. Do not freeze! Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Molecular Weight
17,946 Da
3D Structure
ModBase 3D Structure for P25094
Performance
Cultured human CEM were stimulated overnight in the presence of PMA (10ng/ml) and ionomycin (1uM). Five x 105 cells per tube were incubated 45 minutes on ice with 80 ul of anti-CD25/R-PE at a dilution factor of 1:50 (5 ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.24 log10 fluorescent units when compared to a Mouse IgG1/APC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD25 antibody.
Conjugation
APC
Production
Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.25 mg/ml with an A650/A280 ratio of 1.48.
Buffer
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen
Stimulated PBMC
Applications Tested/Suitable for anti-CD25 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
Application Notes for anti-CD25 antibody
1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)

Product References and Citations for anti-CD25 antibody

(1) L.A. Rubin, et al, (1985) Hybidoma 4: 91-102. (2) B. Boutin, et al, (1989) Neuropediatrics 20: 202-206. (3) T.A. Waldmann, et al, (1989) Annu Rev Biochem 58: 875-911. (4) T.A. Waldmann, et al, (1995) Blood 86: 4063-4075. (5) U.S. Patent No. 4,707,443. (6)Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 403-406.

Other Notes

Small volumes of anti-CD25 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD25 purchase

MBS666125 is an antibody targeting CD25. Also known as anti-human CD25-APC. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD25. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
NCBI Official Full Name
CD25
UniProt Protein Name
Peripheral myelin protein 22
UniProt Synonym Protein Names
Protein CD25; SR13 myelin protein; Schwann cell membrane glycoprotein; SAG
UniProt Gene Name
Pmp22
UniProt Synonym Gene Names
Cd25; Pmp-22; PMP-22; SAG
UniProt Entry Name
PMP22_RAT

UniProt Comments for CD25

Might be involved in growth regulation, and in myelinization in the peripheral nervous system.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD25 antibody

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000