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CD16 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666134

Information: Human CD16 is a low affinity receptor for complexed IgG and occurs in two distinct forms, as a glycosyl phosphtidyl inositol (GPI) anchored protein in granulocytes, and as an integral membrane protein on NK cells and macrophages. Antibody 3G8… More details

$435 / 120 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD16, Monoclonal Antibody
Full Product Name
anti-human CD16-FITC
Product Gene Name
anti-CD16 antibody
Product Synonym Names
Monoclonal anti-human CD16(FcgammaRIII)/FITC
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3G8
Host
Mouse
Species Reactivity
Human, Monkey
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Concentration
Volume: 0.2ml (lot specific)
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended
Molecular Weight
29,089 Da
OMIM
615707
Performance
Five x 105 ficoll prepared human peripheral blood mononuclear cells were washed and incubated 45 minutes on ice with 80 mul anti-CD16/FITC at a 1:50 dilution factor (10 mug/ml). Cells were washed three times, fixed and analyzed by FACS. A 17% sub population of the cells stained positive with a mean shift of 1.67 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 mul of 0.5 mg/ml anti-CD16 antibody.
Conjugation
FITC
Production
Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at 0.5 mg/ml with a Fluorescein/IgG molar ratio of 5.8.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen
Human polymorphonuclear leukocytes
Applications Tested/Suitable for anti-CD16 antibody
Flow Cytometry (FC/FACS), (Not WB)
Application Notes for anti-CD16 antibody
1:50 (or use 1.6mul of concentrated stock per 5 x 105-cell test)

Testing Data of anti-CD16 antibody

Mouse Anti-CD16 Monoclonal Antibody – Cat# MBS666134

Product References and Citations for anti-CD16 antibody

H.B. Fleit, et al, (1982) Proc Natl Acad Sci USA 79: 3275-3279. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 574-597. G. Trinchieri & N. Valiante, (1993) Natural Immunity 12: 218-234. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 805-814. J.C. Edberg & R.P. Kimberly, (1997) J Immunol 159: 3849-3857.

Other Notes

Small volumes of anti-CD16 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD16 purchase

MBS666134 is an antibody targeting CD16. Also known as anti-human CD16-FITC. Reactive in Human, Monkey. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD16. It may not necessarily be applicable to this product.

NCBI GI #
8176551
NCBI GeneID
2214
NCBI Accession #
AAB33925.2
UniProt Secondary Accession #
Q53FJ0; Q53FL6; Q5EBR4; Q65ZM6; Q6PIJ0; A2N6W9
UniProt Related Accession #
P08637
NCBI Official Full Name
CD16, partial
NCBI Official Synonym Full Names
Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
NCBI Official Symbol
FCGR3A
NCBI Official Synonym Symbols
CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor III-A
UniProt Synonym Protein Names
CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; CD_antigen: CD16a
Protein Family
CD160 antigen
UniProt Gene Name
FCGR3A
UniProt Synonym Gene Names
CD16A; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa
UniProt Entry Name
FCG3A_HUMAN
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor III-A; FcgammaRIIIA; CD16a antigen; fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; igG Fc receptor III-2; Fc gamma receptor III-A; Fc-gamma receptor IIIb (CD16); neutrophil-specific antigen NA; Fc-gamma receptor III-2 (CD 16); immunoglobulin G Fc receptor III; Fc fragment of IgG, low affinity III, receptor for (CD16)

NCBI Summary for CD16

This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD16

FCGR3A: Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: IgG binding

Biological Process: regulation of immune response; innate immune response; immune response

Disease: Immunodeficiency 20
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Research Articles on CD16

1. No clear relationship was found between FCGR3A 158V/F polymorphism and response to frontline R-CHOP therapy in patients with diffuse large B-cell lymphoma. [Meta-analysis]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD16 antibody

 Reference Product  PubMed Publications
 SYK antibody  >2 publications with CD16 and SYK
 TYROBP antibody  >1 publications with CD16 and TYROBP

Pathways associated with anti-CD16 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 FCGR Activation Pathway antibodies  FCGR Activation Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway antibodies  Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies  Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Leishmaniasis Pathway antibodies  Leishmaniasis Pathway Diagram
 Leishmaniasis Pathway antibodies  Leishmaniasis Pathway Diagram

Diseases associated with anti-CD16 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >27 publications with CD16 and Necrosis
 Intestinal Diseases Antibodies  >26 publications with CD16 and Intestinal Diseases
 Lupus Erythematosus, Systemic Antibodies  >25 publications with CD16 and Lupus Erythematosus, Systemic
 Cardiovascular Diseases Antibodies  >23 publications with CD16 and Cardiovascular Diseases
 Inflammation Antibodies  >16 publications with CD16 and Inflammation
 Leukemia Antibodies  >14 publications with CD16 and Leukemia
 Breast Neoplasms Antibodies  >12 publications with CD16 and Breast Neoplasms
 Kidney Diseases Antibodies  >12 publications with CD16 and Kidney Diseases
 Neoplasms, Experimental Antibodies  >10 publications with CD16 and Neoplasms, Experimental
 Carcinoma Antibodies  >8 publications with CD16 and Carcinoma

Organs/Tissues associated with anti-CD16 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >153 publications with CD16 and Blood
 Kidney Antibodies  >8 publications with CD16 and Kidney
 Ovary Antibodies  >6 publications with CD16 and Ovary
 Lung Antibodies  >5 publications with CD16 and Lung
 Liver Antibodies  >4 publications with CD16 and Liver
 Brain Antibodies  >3 publications with CD16 and Brain
 Prostate Antibodies  >1 publications with CD16 and Prostate
 Spleen Antibodies  >1 publications with CD16 and Spleen
 Connective Tissue Antibodies  >1 publications with CD16 and Connective Tissue
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