Mouse Anti-CD154 Monoclonal Antibody – Cat# MBS666136

CD154 Antibody – Mouse Monoclonal

Name: CD154 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS666136
Price: 570 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666136

Information: Human CD154 (CD40 Ligand) is a member of the tumor necrosis factor (TNF) family and is expressed on the surface of activated T cells. It can undergo proteolytic cleavage into an immunologically active soluble form. Interaction of CD154 and CD40… More details

$570 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD154, Monoclonal Antibody

Full Product Name

anti-human CD154-Biotin

Product Gene Name

anti-CD154 antibody

Product Synonym Names

Monoclonal anti- human CD154 (CD40 Ligand)/Biotin

Clonality

Monoclonal

Isotype

IgG1

Clone Number

24-31

Host

Mouse

Species Reactivity

Human, Primate

Form/Format

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.

Concentration

1.0 mg/ml (lot specific)

Preparation and Storage

Store at 2 - 5 degree C. Freeze/thawing not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.

Performance

Human Jurkat-4G cells (a CD154 positive subclone of Jurkat) were stimulated 6 hours in the presence of 10 ng/ml PMA and 1 uM Ionomicin. Cells were harvested and washed. Five x 105 cells per tube were washed and incubated 45 minutes on ice with 80 ul of anti-CD154/Biotin at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-PE after which they were washed three times, fixed and analyzed by FACS. A subpopulation of the cells stained positive with a mean shift of 1.25 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD154 antibody.

Conjugation

Biotin

Production

Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHBiotin. Unconjugated Biotin was removed from conjugate using a desalting column.

Buffer

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).

Immunogen

Human sgp39 fusion protein

Applications Tested/Suitable for anti-CD154 antibody

Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunohistchemistry (IHC)

Product References and Citations for anti-CD154 antibody

1) D. Gray, et al, (1994) Seminars in Immunol 6: 303-310. 2) A.C. Grammer, et al, (1995) J Immunol 154: 4996-5010. 3) F. Pietravalle, et al, (1996) J Biol Chemistry 271: 5965-5967. 4) R.J. Noelle, (1996) Immunity 4: 415-419. 5) A. Desai-Mehta, et al, (1996) J Clin Invest 97: 2063-2073. 6) I.S. Grewal and R.A. Flavell, (1996) Immunol Today 17: 410-414. 7) F. Mach, et al, (1997) Proc Natl Acad Sci USA 94:1931-1936. 8) A.C. Grammer, et al, (1999) J Immunol 163: 4150-4159. 9) D. Hollenbaugh, (1992) EMBO 11: 4314-4321. 10) R.K. Vakkalanka, et al, (1999) Arthritis Rhem 42:871-81. 11) M. Jinnin, et al.(2003) Ann Rhem Dis 62: 190-191. 12) U. Schonbeck, et al, (2000) PNAS USA 97: 7458-7463. 13) U. Schonbeck, et al, (2001) Circulation 104: 2266-2268.

Other Notes

Small volumes of anti-CD154 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD154 purchase

MBS666136 is an antibody targeting CD154. Also known as anti-human CD154-Biotin. Reactive in Human, Primate. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD154. It may not necessarily be applicable to this product.

Protein Family

CD40 ligand

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.