Mouse Anti-MHC Monoclonal Antibody – Cat# MBS666184

Class I (MHC) Antibody – Mouse Monoclonal

Name: MHC Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS666184
Price: 380 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666184

Information: Human MHC Class I (HLA-A, B & C) molecules are expressed on nearly all nucleated cells. MHC Class I molecules consist of heterodimers of a highly polymorphic transmembrane alpha chain and an invariant β chain. MHC Class I molecules bind to… More details

$380 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MHC Class I, Monoclonal Antibody

Full Product Name

anti-human MHC Class I-Purified (with Antibiotics)

Product Synonym Names

Monoclonal anti-human MHC Class I (HLA-A, B and C)

Clonality

Monoclonal

Isotype

Mouse IgG2a

Clone Number

3F10

Host

Mouse

Species Reactivity

Human

Form/Format

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate (as a preservative).

Concentration

1.0 mg/ml (lot specific)

Preparation and Storage

Store at 2 - 5 degree C. Freeze/Thawing is not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.

Performance

Five x 105 cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80 ul of anti-MHC Class I antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, (MBS666783) after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.17 log10 fluorescent units when compared to a Mouse IgG2a isotype control (MBS666112) at a similar concentration.

Production

Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE with less than 1% Bovine Immunoglobulin.

Immunogen

Human HSB-2 T cell line

Product References and Citations for anti-MHC Class I antibody

G.S. Eisenbarth, et al, (1980) J Immunol 124: 1237-1244. P.J. Bjorkman & P. Parham, (1990) Annu Rev Biochem 59: 253-288. L.D. Barber & P. Parham, (1994) J Exp Med 180: 1191-1194. K.L. Rock, (1996) Immunol Today 17: 131-137.

Other Notes

Small volumes of anti-MHC Class I antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MHC Class I purchase

MBS666184 is an antibody targeting MHC Class I. Also known as anti-human MHC Class I-Purified (with Antibiotics). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MHC Class I. It may not necessarily be applicable to this product.

NCBI GI #

1545844

NCBI Accession #

AAB08079.1

NCBI Official Full Name

MHC class I, partial

UniProt Protein Name

MHC class I

UniProt Synonym Protein Names

MHC class I

Protein Family

MHC class I polypeptide-related sequence

UniProt Entry Name

Q95430_PONPY

UniProt Comments for MHC Class I

Involved in the presentation of foreign antigens to the immune system.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.