CD45R Antibody – Rat Monoclonal

Monoclonal

IgM

RA3-3A1/6.1

Rat

Mouse

50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate

Purified (with Antibiotics)

1.0 mg/ml (lot specific)

Store at 2 - 5 degree C. Freeze/Thawing is not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.

Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20ul of 300ug/ml murine IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-B220 antibody diluted to 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Rat IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. An 11.3% sub population of the cells stained positive with a mean shift of 1.5 log10 fluorescent units when compared to background.

Antibody was purified from tissue culture supernatant by size exclusion chromatography. Purity was >90% Immunoglobulin by SDS-PAGE.

50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).

anti-mouse CD45R(B220)

RAW112 cells