• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CD11b Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666198

Information: Human CD11b (alpha M integrin) complexes with CD18 (beta 2 integrin) to form the complement receptor type 3 (CR3) heterodimer which binds to ICAM-1, fibrinogen, C3b, and coagulation factor X. CD11b expression is restricted to leukocytes, mainly… More details

$435 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD11b, Monoclonal Antibody
Full Product Name
anti-human CD11b-Biotin
Product Gene Name
anti-CD11b antibody
Product Synonym Names
Monoclonal anti-human CD11b(CR3, Mac-1, integrin aM)/Biotin
Clonality
Monoclonal
Isotype
IgG1
Clone Number
ICRF44
Host
Mouse
Species Reactivity
Human, Primate, Pig, Monkey
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
Store at 2 - 5 degree C. Freeze/thawing not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Molecular Weight
102,441 Da
Performance
Five x 105 ficoll prepared human peripheral white blood cells were washed and preincubated ~ 10 minutes with 20 ul human IgG at 250 ug/ml (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD11b/Biotin at a concentration of 10 ug/ml. Cells were then washed twice and incubated 45 minutes with 2 degree reagent Streptavidin/R-PE, after which they were washed three times, fixed and analyzed using a BD FACstar plus. A net 19% sub population of the cells stained positive with a mean shift of 1.69 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was 94% blocked when cells were preincubated ~10 minutes with 20 ul of 0.5 mg/ml unlabeled anti-CD11b antibody.
Conjugation
Biotin
Production
Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative)
Immunogen
Human monocytes
Applications Tested/Suitable for anti-CD11b antibody
Flow Cytometry (FC/FACS)

Testing Data of anti-CD11b antibody

Mouse Anti-CD11b Monoclonal Antibody – Cat# MBS666198

Product References and Citations for anti-CD11b antibody

V. Mathotra, et al, (1986) Eur J Immunol 16: 1117-1123. B.L. Myones, et al, (1988) J Clin Invest 82: 640-651. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 555-558, 560-562. H.R. Petty & R.F. Todd, (1993) J Leuk Biol 54: 492-494. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1588-1590.

Other Notes

Small volumes of anti-CD11b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD11b purchase

MBS666198 is an antibody targeting CD11b. Also known as anti-human CD11b-Biotin. Reactive in Human, Primate, Pig, Monkey. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD11b. It may not necessarily be applicable to this product.

NCBI GI #
1616950
NCBI GeneID
397459
NCBI Accession #
AAB16869.1
NCBI Official Full Name
CD11b, partial
NCBI Official Symbol
ITGAM
NCBI Official Synonym Symbols
CD11B
UniProt Protein Name
CD11b
UniProt Synonym Protein Names
CD11b
Protein Family
Integrin
UniProt Gene Name
CD11b
UniProt Entry Name
Q28984_PIG
NCBI Protein Information
integrin alpha-M

Research Articles on CD11b

1. molecular cloning, characterization, gene expression and chromosomal localization on region SSC3p15-17

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD11b antibody

 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000