Mouse Anti-CD28 Monoclonal Antibody – Cat# MBS666199

CD28 Antibody – Mouse Monoclonal

Name: CD28 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS666199
Price: 605 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666199

Information: Human CD28 is an important costimulatory molecule found on all CD4+ T cells and on about half of the CD8+ T cells. T cell activities attributed to CD28 include prevention of anergy, induction of cytokine gene transcription, stabilization of… More details

$605 / 120 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD28, Monoclonal Antibody

Full Product Name

anti-human CD28-R-PE

Product Gene Name

anti-CD28 antibody

Product Synonym Names

Monoclonal anti-human CD28/R-PE

Clonality

Monoclonal

Isotype

IgG1k

Clone Number

ANC28.1/5D10

Host

Mouse

Species Reactivity

Human

Form/Format

50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA.

Concentration

Volume: 0.2 ml (lot specific)

Preparation and Storage

Store at 2 - 5 degree C. Do not freeze! Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.

Molecular Weight

25,243 Da

Performance

Five x 105 cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD28/R-PE at a 1:50 dilution (20ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.88 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD28 antibody.

Conjugation

Production

Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 1.0 mg/ml with an A565/A280 ratio of 3.03.

Buffer

50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).

Immunogen

Recombinant Human CD28 and HPB-MLT human tumor cells

Applications Tested/Suitable for anti-CD28 antibody

Flow Cytometry (FC/FACS), ELISA (EIA), Agonist

Application Notes for anti-CD28 antibody

1:50 (or use 1.6 ul of concentrated stock per 5 x 105-cell test)

Product References and Citations for anti-CD28 antibody

(1) M.K. Jenkins & J.G. Johnson, (1993) Curr Opin Immunol 5: 361-367. (2) 61st Forum in Immunology, (1995) Res Immunol 146: 127-205. (3) I. Kariv, A. Truneh & R.W. Sweet, (1996) J Immunol 157: 29-38. (4) J. Carling, et al, (2003) J Immunol 33: 135-142.

Other Notes

Small volumes of anti-CD28 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD28 purchase

MBS666199 is an antibody targeting CD28. Also known as anti-human CD28-R-PE. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD28. It may not necessarily be applicable to this product.

NCBI GI #

192485

NCBI GeneID

12487

NCBI Accession #

AAA37396.1

UniProt Secondary Accession #

Q6GSH7

UniProt Related Accession #

P31041

NCBI Official Full Name

CD28, partial

NCBI Official Synonym Full Names

CD28 antigen

NCBI Official Symbol

Cd28

UniProt Protein Name

T-cell-specific surface glycoprotein CD28

UniProt Synonym Protein Names

CD_antigen: CD28

Protein Family

T-cell-specific surface glycoprotein

UniProt Gene Name

Cd28

UniProt Entry Name

CD28_MOUSE

NCBI Protein Information

T-cell-specific surface glycoprotein CD28

UniProt Comments for CD28

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Cell surface; Membrane protein, integral

Cellular Component: membrane; plasma membrane; integral to membrane; immunological synapse; lipid raft; external side of plasma membrane

Molecular Function: protein homodimerization activity; SH3/SH2 adaptor activity; protease binding

Biological Process: positive regulation of isotype switching to IgG isotypes; T cell activation; positive regulation of mitosis; positive regulation of interleukin-2 biosynthetic process; negative thymic T cell selection; T cell receptor signaling pathway; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of inflammatory response to antigenic stimulus; positive regulation of protein kinase B signaling cascade; T cell costimulation; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell proliferation; immune response; positive regulation of transcription from RNA polymerase II promoter; regulatory T cell differentiation

Research Articles on CD28

1. CD28 costimulation is essential for conferring host protection during secondary N. brasiliensis infection.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD28 antibody

Reference Product	PubMed Publications
Cd80 antibody	>1802 publications with CD28 and Cd80
Cd86 antibody	>1077 publications with CD28 and Cd86
Lck antibody	>166 publications with CD28 and Lck
Grb2 antibody	>29 publications with CD28 and Grb2
Grap2 antibody	>6 publications with CD28 and Grap2
Pik3r3 antibody	>2 publications with CD28 and Pik3r3

Pathways associated with anti-CD28 antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
CD28 Co-stimulation Pathway antibodies	CD28 Co-stimulation Pathway Diagram
CD28 Dependent PI3K/Akt Signaling Pathway antibodies	CD28 Dependent PI3K/Akt Signaling Pathway Diagram
CD28 Dependent Vav1 Pathway antibodies	CD28 Dependent Vav1 Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram

Diseases associated with anti-CD28 antibody

Disease Name	Pubmed Publications
Autoimmune Diseases Antibodies	>1223 publications with CD28 and Autoimmune Diseases
Necrosis Antibodies	>840 publications with CD28 and Necrosis
Inflammation Antibodies	>785 publications with CD28 and Inflammation
HIV Infections Antibodies	>452 publications with CD28 and HIV Infections
Arthritis, Rheumatoid Antibodies	>356 publications with CD28 and Arthritis, Rheumatoid
Lung Diseases Antibodies	>310 publications with CD28 and Lung Diseases
Lymphoma Antibodies	>281 publications with CD28 and Lymphoma
Carcinoma Antibodies	>229 publications with CD28 and Carcinoma
Neoplasms, Experimental Antibodies	>208 publications with CD28 and Neoplasms, Experimental
Liver Diseases Antibodies	>162 publications with CD28 and Liver Diseases

Organs/Tissues associated with anti-CD28 antibody

Organ/Tissue Name	Pubmed Publications
Spleen Antibodies	>554 publications with CD28 and Spleen
Bone Antibodies	>414 publications with CD28 and Bone
Bone Marrow Antibodies	>368 publications with CD28 and Bone Marrow
Thymus Antibodies	>338 publications with CD28 and Thymus
Lung Antibodies	>336 publications with CD28 and Lung
Skin Antibodies	>312 publications with CD28 and Skin
Joint Antibodies	>148 publications with CD28 and Joint
Embryonic Tissue Antibodies	>56 publications with CD28 and Embryonic Tissue
Mammary Gland Antibodies	>3 publications with CD28 and Mammary Gland