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CD58 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666227

Information: Human CD58 is a cell surface glycoprotein that functions as an adhesion molocule and mediates costimulatory activity through its ligand CD2, which is expressed on T and natural killer cells (1,2). CD58 has wide cellular distribution on both… More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD58, Monoclonal Antibody
Full Product Name
anti-human CD58(LFA-3)-Purified Preservative Free
Product Gene Name
anti-CD58 antibody
Product Synonym Names
Monoclonal anti-human CD58(LFA-3)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
TS2
Host
Mouse
Species Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
Purity/Purification
Purified Preservative Free
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
Store at 2 - 5 degree C. Open under aseptic conditions. Freeze/Thawing is not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Molecular Weight
27,960 Da
OMIM
153420
3D Structure
ModBase 3D Structure for P19256
Performance
Five x 105 cultured Nalm-6 cells were washed after which they were incubated 45 minutes on ice with 80 ul of anti-CD58 antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.46 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Production
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE with less than 1% Bovine Immunoglobulin.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. Product was sterile filtered under aseptic conditions.
Immunogen
Human cytotoxic  T cells
Applications Tested/Suitable for anti-CD58 antibody
Flow Cytometry (FC/FACS)

Testing Data of anti-CD58 antibody

Mouse Anti-CD58 Monoclonal Antibody – Cat# MBS666227

Product References and Citations for anti-CD58 antibody

(1) Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 414-415. (2) A.R. Arulanandam, et al, (1994) J Exp Med 180:1861-1871.

Other Notes

Small volumes of anti-CD58 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD58 purchase

MBS666227 is an antibody targeting CD58. Also known as anti-human CD58(LFA-3)-Purified Preservative Free. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD58. It may not necessarily be applicable to this product.

NCBI GI #
48145995
NCBI GeneID
965
NCBI Accession #
CAG33220.1
UniProt Primary Accession #
P19256
UniProt Secondary Accession #
Q5U053; Q6IB65; Q96KI9; A8K7G5
UniProt Related Accession #
P19256
NCBI Official Full Name
CD58
NCBI Official Synonym Full Names
CD58 molecule
NCBI Official Symbol
CD58
NCBI Official Synonym Symbols
ag3; LFA3; LFA-3
UniProt Protein Name
Lymphocyte function-associated antigen 3
UniProt Synonym Protein Names
Surface glycoprotein LFA-3; CD_antigen: CD58
Protein Family
Lymphocyte function-associated antigen
UniProt Gene Name
CD58
UniProt Synonym Gene Names
LFA3; Ag3
UniProt Entry Name
LFA3_HUMAN
NCBI Protein Information
lymphocyte function-associated antigen 3; surface glycoprotein LFA-3; CD58 antigen, (lymphocyte function-associated antigen 3)

NCBI Summary for CD58

This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]
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UniProt Comments for CD58

CD58: Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen- presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, GPI anchor; Cell adhesion

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: cell surface; membrane; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: heterotypic cell-cell adhesion; cell-cell adhesion; cell adhesion; blood coagulation; leukocyte migration
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Research Articles on CD58

1. Genetic variations in CD58 were associated with the susceptibility of neuromyelitis optica in a Korean population.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD58 antibody

 Reference Product  PubMed Publications
 CD2 antibody  >416 publications with CD58 and CD2
 ICAM1 antibody  >288 publications with CD58 and ICAM1
 ITGAL antibody  >138 publications with CD58 and ITGAL
 CD86 antibody  >108 publications with CD58 and CD86
 CD40 antibody  >101 publications with CD58 and CD40
 IFNA17 antibody  >8 publications with CD58 and IFNA17
 CD2AP antibody  >1 publications with CD58 and CD2AP

Pathways associated with anti-CD58 antibody

 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram

Diseases associated with anti-CD58 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >110 publications with CD58 and Necrosis
 Inflammation Antibodies  >42 publications with CD58 and Inflammation
 Nervous System Diseases Antibodies  >42 publications with CD58 and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >40 publications with CD58 and Neoplasms, Experimental
 Cardiovascular Diseases Antibodies  >27 publications with CD58 and Cardiovascular Diseases
 Anemia Antibodies  >23 publications with CD58 and Anemia
 Multiple Sclerosis Antibodies  >23 publications with CD58 and Multiple Sclerosis
 Liver Diseases Antibodies  >22 publications with CD58 and Liver Diseases
 Cell Transformation, Neoplastic Antibodies  >21 publications with CD58 and Cell Transformation, Neoplastic
 Disease Models, Animal Antibodies  >14 publications with CD58 and Disease Models, Animal

Organs/Tissues associated with anti-CD58 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >746 publications with CD58 and Blood
 Bone Antibodies  >84 publications with CD58 and Bone
 Bone Marrow Antibodies  >80 publications with CD58 and Bone Marrow
 Skin Antibodies  >41 publications with CD58 and Skin
 Lung Antibodies  >33 publications with CD58 and Lung
 Liver Antibodies  >30 publications with CD58 and Liver
 Brain Antibodies  >24 publications with CD58 and Brain
 Embryonic Tissue Antibodies  >20 publications with CD58 and Embryonic Tissue
 Kidney Antibodies  >18 publications with CD58 and Kidney
 Lymph Node Antibodies  >17 publications with CD58 and Lymph Node
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