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CD32 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666253

Information: Human CD32 exists in at least six isoforms originating from three different genes (Fcƒ×RII A, B and C). The CD32 molecule is a low affinity receptor for immune complexed IgG and has signal-transducing capabilities involved with humoral and… More details

$1260 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD32, Monoclonal Antibody
Full Product Name
anti-human CD32-Fab
Product Gene Name
Product Synonym Names
Monoclonal anti-human CD32(Fc gamma RII) Fab
Clonality
Monoclonal
Isotype
IgG1k
Clone Number
7.3
Host
Mouse
Species Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. Product was sterile filtered and vialed under aseptic conditions. No preservative added.
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
Store at 2 - 5°C. Open under aseptic conditions. Freeze/Thawing is not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Performance
Five x 105 cultured human Raji cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD32 Fab at 10 ug/ml. Cells were washed twice and incubated with 2° reagent Goat anti-Mouse IgG/FITC (Catalog # MBS666783 ), after which they were washed three times, fixed and analyzed by FC. Cells stained positive with a mean shift of 0.55 log10 fluorescent units when compared to a Mouse IgG1 Fab’ negative control (Catalog # MBS666135 ).
Conjugation
Fab
Production
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Immunoglobulin was enzymaticly cleaved using Immobilized Ficin. Fab was separated from intact antibody and Fc fragments by Protein A and Size exclusion chromatography. No intact antibody was observed by SDS-PAGE.
Immunogen
Human K562 tumor cells and L cells transfected with human Fc gamma RII
Applications Tested/Suitable for anti-CD32 antibody
Flow Cytometry (FC)

Testing Data of anti-CD32 antibody

Mouse Anti-CD32 Monoclonal Antibody – Cat# MBS666253

Product References and Citations for anti-CD32 antibody

Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 823-836.

Other Notes

Small volumes of anti-CD32 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD32 purchase

MBS666253 is an antibody targeting CD32. Also known as anti-human CD32-Fab. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD32. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
CD32
Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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