Mouse Anti-CD357 Monoclonal Antibody – Cat# MBS666262

CD357 Antibody – Mouse Monoclonal

Name: CD357 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS666262
Price: 435 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666262

Information: Glucocorticoid induced Tumor Necrosis Factor receptor family related gene (GITR, AITR) is a type 1 228 amino acid transmembrane protein that has been designated TNFRSF18. GITR is expressed mainly by CD4+ CD25+ T lymphocytes. Its engagement by… More details

$435 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD357, Monoclonal Antibody

Full Product Name

anti-human CD357(GITR)-Purified Preservative Free

Product Gene Name

anti-CD357 antibody

Product Synonym Names

Monoclonal anti-human CD357 (GITR, AITR)

Clonality

Monoclonal

Isotype

IgG3k

Clone Number

ANC5E3

Host

Mouse

Species Reactivity

Human

Form/Format

50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl

Purity/Purification

Purified Preservative Free

Concentration

1.0 mg/ml (lot specific)

Preparation and Storage

Store at 2 - 5 degree C. Open under aseptic conditions. Freeze/Thawing is not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.

Performance

Ficoll prepared human peripheral blood leukocytes (PBL) were stimulated 5 days in culture with PHA. Five x 105 cells per tube were washed and pre incubated 5 minutes with 20 ul of 250 ug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-GITR mAb at a concentration of 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat-anti-Mouse Ig/FITC, after which they were washed three times and analyzed by FACS. Cells stained positive with a mean shift of 0.52 log10 fluorescent units when compared to a Mouse IgG3 negative control at a similar concentration.

Production

Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2 um filtered and vialed under aseptic conditions.

Buffer

50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl.

Immunogen

Recombinant human GITR, PHA stimulated human PBL

Applications Tested/Suitable for anti-CD357 antibody

Flow Cytometry (FC/FACS), ELISA (EIA)

Product References and Citations for anti-CD357 antibody

1) Gurney AL, et al. (1999) Curr Biol 9(4): 215-218. 2) Ji HB, et al. (2004) J Immunol 172(10): 5823-5827. 3) Kim WJ, WH Lee, et al. (2006) Immunology 119(3): 44-9. 4) Furze RC, Selkirk ME, et al. (2006) Microbes Infect 8(12-13): 2803-10. 5) Kohm AP, SD Miller, et al. (2004) J Immunol 172: 4686-4690. 6) McHugh, R.S. et al. (2002) Immunity 16:311. 7) Shin, H.H. et al. (2002) FEBS Lett. 514:275. 8). Shimizu, J. et al. (2002) Nature Immunol. 3:135.

Other Notes

Small volumes of anti-CD357 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD357 purchase

MBS666262 is an antibody targeting CD357. Also known as anti-human CD357(GITR)-Purified Preservative Free. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD357. It may not necessarily be applicable to this product.

Protein Family

Tumor necrosis factor receptor superfamily

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.