Mouse Anti-CD94 Monoclonal Antibody – Cat# MBS666268

CD94 Antibody – Mouse Monoclonal

Name: CD94 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS666268
Price: 570 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666268

Information: Human CD94 expression is restricted to natural killer (NK) cells and a small subset of T lymphocytes. CD94 is a type II membrane glycoprotein of the c-type lectin superfamily and essentially lacks a cytoplasmic tail. Antibody HP-3D9 recognizes… More details

$570 / 120 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD94, Monoclonal Antibody

Full Product Name

anti-human CD94-FITC

Product Gene Name

anti-CD94 antibody

Product Synonym Names

Monoclonal anti-human CD94 (Kp43)/FITC

Clonality

Monoclonal

Isotype

IgG1

Clone Number

HP-3D9

Host

Mouse

Species Reactivity

Human

Form/Format

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.

Concentration

Volume: 100 ul (lot specific)

Preparation and Storage

Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light
Product should retain activity for at least 12 months after shipping date when stored as recommended.

Molecular Weight

17,109 Da

OMIM

602894

3D Structure

ModBase 3D Structure for Q13241

Performance

Ficoll prepared human peripheral white blood cells were stimulated by incubating 1 day at 5 x 106 cells/ml in RPMI 10% FBS media including 5 mug/ml Phytohemagglutinin-P. Five x 105 cells per tube were then washed and incubated 45 minutes on ice with 80 mul of anti-CD94/FITC at a 1:100 dilution (5mug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.09 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 mul of 0.5 mg/ml anti-CD94 antibody.

Conjugation

FITC

Production

Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at 0.25 mg/ml with a Fluorescein/IgG molar ratio of 8.6.

Buffer

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).

Immunogen

Cultured human NK cells

Applications Tested/Suitable for anti-CD94 antibody

Flow Cytometry (FC/FACS)

Application Notes for anti-CD94 antibody

1:100 (or use 0.8 mul of concentrated stock per 5 x 105-cell test)

Product References and Citations for anti-CD94 antibody

J. Aramburu, et al, (1990) J Immunol 144: 3238-3247. J. Aramburu, et al, J Immunol (1991) 147: 714-721. A. Moretta, et al, J Exp Med (1994) 180: 545-555. C. Chang, et al, Eur J Immunol (1995) 25: 2433-2437. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p.1437-1439.

Other Notes

Small volumes of anti-CD94 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD94 purchase

MBS666268 is an antibody targeting CD94. Also known as anti-human CD94-FITC. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD94. It may not necessarily be applicable to this product.

NCBI GI #

2995204

NCBI GeneID

3824

NCBI Accession #

CAA74663.1

UniProt Primary Accession #

Q13241

UniProt Secondary Accession #

O43321; O43773; Q9UBE3; Q9UEQ0

UniProt Related Accession #

Q13241

NCBI Official Full Name

CD94, partial

NCBI Official Synonym Full Names

killer cell lectin-like receptor subfamily D, member 1

NCBI Official Symbol

KLRD1

NCBI Official Synonym Symbols

CD94

UniProt Protein Name

Natural killer cells antigen CD94

UniProt Synonym Protein Names

KP43; Killer cell lectin-like receptor subfamily D member 1; NK cell receptor; CD_antigen: CD94

Protein Family

Natural killer cells antigen

UniProt Gene Name

KLRD1

UniProt Synonym Gene Names

CD94

UniProt Entry Name

KLRD1_HUMAN

NCBI Protein Information

natural killer cells antigen CD94; KP43; CD94 antigen; NK cell receptor

NCBI Summary for CD94

Natural killer (NK) cells are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for CD94

KLRD1: Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: plasma membrane; integral to membrane; receptor complex; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; carbohydrate binding

Biological Process: regulation of immune response; cell surface receptor linked signal transduction; natural killer cell mediated immunity; innate immune response

Research Articles on CD94

1. Data indicate that NKG2 receptor NKG2E was capable of associating with CD94 and DAP12 but that the complex was retained intracellularly at the endoplasmic reticulum.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD94 antibody

Reference Product	PubMed Publications
KLRC1 antibody	>165 publications with CD94 and KLRC1
HLA-E antibody	>112 publications with CD94 and HLA-E
KLRC2 antibody	>62 publications with CD94 and KLRC2
HLA-C antibody	>28 publications with CD94 and HLA-C
KLRC3 antibody	>18 publications with CD94 and KLRC3
TYROBP antibody	>7 publications with CD94 and TYROBP

Pathways associated with anti-CD94 antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Antigen Processing And Presentation Pathway antibodies	Antigen Processing And Presentation Pathway Diagram
Antigen Processing And Presentation Pathway antibodies	Antigen Processing And Presentation Pathway Diagram
DAP12 Interactions Pathway antibodies	DAP12 Interactions Pathway Diagram
DAP12 Signaling Pathway antibodies	DAP12 Signaling Pathway Diagram
Graft-versus-host Disease Pathway antibodies	Graft-versus-host Disease Pathway Diagram
Graft-versus-host Disease Pathway antibodies	Graft-versus-host Disease Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies	Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram

Diseases associated with anti-CD94 antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>24 publications with CD94 and Necrosis
Skin Diseases Antibodies	>16 publications with CD94 and Skin Diseases
Inflammation Antibodies	>15 publications with CD94 and Inflammation
Neoplasms, Experimental Antibodies	>13 publications with CD94 and Neoplasms, Experimental
Disease Models, Animal Antibodies	>9 publications with CD94 and Disease Models, Animal
Cardiovascular Diseases Antibodies	>8 publications with CD94 and Cardiovascular Diseases
Nervous System Diseases Antibodies	>7 publications with CD94 and Nervous System Diseases
Liver Diseases Antibodies	>7 publications with CD94 and Liver Diseases
Lung Diseases Antibodies	>4 publications with CD94 and Lung Diseases
Skin Neoplasms Antibodies	>4 publications with CD94 and Skin Neoplasms

Organs/Tissues associated with anti-CD94 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>449 publications with CD94 and Blood
Placenta Antibodies	>20 publications with CD94 and Placenta
Liver Antibodies	>15 publications with CD94 and Liver
Uncharacterized Tissue Antibodies	>1 publications with CD94 and Uncharacterized Tissue