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CD14 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666359

Information: Human CD14 is strongly expressed on myelomonocyte lineage cells including monocytes, macrophages, and a subset of granulocytes. CD14 is a high affinity receptor for complexes of lipopolysaccharide and lipopolysaccharide binding protein. A soluble… More details

$435 / 120 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD14, Monoclonal Antibody
Full Product Name
anti-human CD14-FITC
Product Gene Name
anti-CD14 antibody
Product Synonym Names
Monoclonal anti-human CD14/FITC
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
UCHM1
Host
Mouse
Species Reactivity
Human, Monkey
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Concentration
Volume: 200 ul (lot specific)
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Molecular Weight
39,667 Da
Performance
Five x 105 ficoll prepared human peripheral blood mononuclear cells were washed and pre incubated 5 minutes with 20mul of 250mug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 mul of anti-CD14/FITC at a 1:50 dilution (10 mug/ml). Cells were washed three times, fixed and analyzed by FACS. A net 9.7% sub population of the cells stained positive with a mean shift of 1.82 log10 fluorescent units when compared to a Mouse IgG2a/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 mul of 0.5 mg/ml anti-CD14 antibody.
Conjugation
FITC
Production
Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to 95% Immunoglobulin by SDS-PAGE, and reacted with FITC. Unconjugated FITC was removed from conjugate using a desalting column. Antibody conjugate is at approximately 0.5 mg/ml with a Fluorescein/Antibody molar ratio of 15.0
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen
Human thymocytes/Sezary T cells
Applications Tested/Suitable for anti-CD14 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD14 antibody
1:50 (or use 1.6mul of concentrated stock per 5 x 105-cell test)

Testing Data of anti-CD14 antibody

Mouse Anti-CD14 Monoclonal Antibody – Cat# MBS666359

Product References and Citations for anti-CD14 antibody

N. Hogg, et al, (1984) Immunology 53: 753-767. M.O. Labeta, et al, (1993) Eur J Immunol 23: 2144-2151. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) "Reference Antibody". Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 778-788. S.D. Wright, (1995) J Immunol 155: 6-8.

Other Notes

Small volumes of anti-CD14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD14 purchase

MBS666359 is an antibody targeting CD14. Also known as anti-human CD14-FITC. Reactive in Human, Monkey. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD14. It may not necessarily be applicable to this product.

NCBI GI #
2274810
NCBI GeneID
281048
NCBI Accession #
BAA21517.1
UniProt Secondary Accession #
Q9TVA7; A8DBS7
UniProt Related Accession #
Q95122
NCBI Official Full Name
CD14
NCBI Official Synonym Full Names
CD14 molecule<
NCBI Official Symbol
CD14
UniProt Protein Name
Monocyte differentiation antigen CD14
UniProt Synonym Protein Names
Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14
Protein Family
Monocyte differentiation antigen
UniProt Gene Name
CD14
UniProt Entry Name
CD14_BOVIN
NCBI Protein Information
monocyte differentiation antigen CD14; CD14 antigen; myeloid cell-specific leucine-rich glycoprotein

UniProt Comments for CD14

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- ().
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Research Articles on CD14

1. Data from an in vitro co-culture model suggest that an early response of endometrium in uterine infection is up-regulation of expression of CD14 and TLR4 (toll-like receptor 4).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD14 antibody

 Reference Product  PubMed Publications
 TLR4 antibody  >1358 publications with CD14 and TLR4
 TLR2 antibody  >717 publications with CD14 and TLR2
 LBP antibody  >426 publications with CD14 and LBP
 LY96 antibody  >147 publications with CD14 and LY96
 RELA antibody  >29 publications with CD14 and RELA
 TICAM2 antibody  >9 publications with CD14 and TICAM2
 NFKB1 antibody  >5 publications with CD14 and NFKB1
 F2R antibody  >1 publications with CD14 and F2R

Pathways associated with anti-CD14 antibody

 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway antibodies  Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 IKK Complex Recruitment Mediated By RIP1 Pathway antibodies  IKK Complex Recruitment Mediated By RIP1 Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram

Diseases associated with anti-CD14 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >2284 publications with CD14 and Necrosis
 Inflammation Antibodies  >2250 publications with CD14 and Inflammation
 Cardiovascular Diseases Antibodies  >629 publications with CD14 and Cardiovascular Diseases
 Lung Diseases Antibodies  >519 publications with CD14 and Lung Diseases
 Liver Diseases Antibodies  >354 publications with CD14 and Liver Diseases
 Nervous System Diseases Antibodies  >351 publications with CD14 and Nervous System Diseases
 Disease Models, Animal Antibodies  >327 publications with CD14 and Disease Models, Animal
 Heart Diseases Antibodies  >271 publications with CD14 and Heart Diseases
 Asthma Antibodies  >258 publications with CD14 and Asthma
 Kidney Diseases Antibodies  >191 publications with CD14 and Kidney Diseases

Organs/Tissues associated with anti-CD14 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7387 publications with CD14 and Blood
 Liver Antibodies  >610 publications with CD14 and Liver
 Kidney Antibodies  >325 publications with CD14 and Kidney
 Brain Antibodies  >299 publications with CD14 and Brain
 Muscle Antibodies  >275 publications with CD14 and Muscle
 Intestine Antibodies  >234 publications with CD14 and Intestine
 Adipose Tissue Antibodies  >145 publications with CD14 and Adipose Tissue
 Uterus Antibodies  >144 publications with CD14 and Uterus
 Ovary Antibodies  >116 publications with CD14 and Ovary
 Reticulum Antibodies  >44 publications with CD14 and Reticulum
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