CD13 Antibody – Mouse Monoclonal

Monoclonal

IgG2ak

22A5

Mouse

Human

50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA.

Volume: 0.2 ml (lot specific)

Store at 2 - 5 degree C. Do not freeze! Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.

Five x 105 cultured THP-1 cells were washed and pre incubated with 20 mul of 250 mug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 mul of anti-CD13/R-PE at a 1:50 dilution (10 mug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.47 log10 fluorescent units when compared to a Mouse IgG2a/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 mul of 0.5 mg/ml anti-CD13 antibody.

PE

Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.5 mg/ml with an A565/A280 ratio of 2.50.

50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).

Human osteosarcoma tissue