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CD83 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666502

Information: Human CD83 is a type 1 cell surface glycoprotein expressed almost solely by dendritic cells that play an important role in antigen presentation (2). Antibody HB15e recognizes the CD83 molecule of 45 kd (3). More details

$435 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD83, Monoclonal Antibody
Full Product Name
anti-human CD83-Biotin
Product Gene Name
Product Synonym Names
Monoclonal anti-human human CD83/Biotin
Clonality
Monoclonal
Isotype
IgG1
Clone Number
HB15e
Host
Mouse
Species Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
Store at 2 - 5 degree C. Freeze/thawing not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Molecular Weight
23,042 Da
OMIM
604534
3D Structure
ModBase 3D Structure for Q01151
Performance
Five x 105 cultured human Raji cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD83/Biotin at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-PE, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.0 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD83 antibody.
Conjugation
Biotin
Production
Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen
COS cells transfected with human CD83
Applications Tested/Suitable for anti-CD83 antibody
Flow Cytometry (FC/FACS)

Testing Data of anti-CD83 antibody

Mouse Anti-CD83 Monoclonal Antibody – Cat# MBS666502

Product References and Citations for anti-CD83 antibody

(1). L-J. Zhou, et al, (1992) J Immunol 149: 735-742. (2). L-J. Zhou & T.F. Tedder (1995) J Immunol 154: 3821-3835. (3). Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 191-193.

Other Notes

Small volumes of anti-CD83 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD83 purchase

MBS666502 is an antibody targeting CD83. Also known as anti-human CD83-Biotin. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD83. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
CD83
NCBI Official Synonym Full Names
CD83 molecule
NCBI Official Symbol
CD83
NCBI Official Synonym Symbols
BL11; HB15
UniProt Protein Name
CD83 antigen
UniProt Synonym Protein Names
B-cell activation protein; Cell surface protein HB15; CD_antigen: CD83
Protein Family
UniProt Gene Name
CD83
UniProt Synonym Gene Names
hCD83
UniProt Entry Name
CD83_HUMAN
NCBI Protein Information
CD83 antigen; hCD83; B-cell activation protein; cell surface protein HB15; cell-surface glycoprotein; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily)

NCBI Summary for CD83

The protein encoded by this gene is a single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The encoded protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
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UniProt Comments for CD83

CD83: May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p23

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Biological Process: negative regulation of interleukin-4 production; positive regulation of interleukin-2 production; defense response; positive regulation of CD4-positive, alpha beta T cell differentiation; signal transduction; response to organic cyclic substance; positive regulation of interleukin-10 production; humoral immune response
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD83 antibody

 Products by Pathway  Pathway Diagram
 TCR Signaling Pathway antibodies  TCR Signaling Pathway Diagram
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