Mouse Anti-CD278 Monoclonal Antibody – Cat# MBS666514

CD278 Antibody – Mouse Monoclonal

Name: CD278 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS666514
Price: 570 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666514

Information: The inducible costimulator CD278 (ICOS, T cell activation molecule H4) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Secondary signaling through CD28 or ICOS results in discrete cytokine… More details

$570 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD278, Monoclonal Antibody

Full Product Name

anti-human CD278(ICOS)-Biotin

Product Gene Name

anti-CD278 antibody

Product Synonym Names

Monoclonal anti-human CD278(ICOS)/Biotin

Clonality

Monoclonal

Isotype

IgG1k

Clone Number

ANC6C6

Host

Mouse

Species Reactivity

Human

Form/Format

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.

Concentration

0.5 mg/ml (lot specific)

Preparation and Storage

Store at 2 - 5 degree C. Freeze/thawing not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.

Performance

Ficoll prepared human peripheral blood mononuclear cells were stimulated 5 days in culture with 5 ug/ml PHA after which they were harvested and washed in FACS buffer. Five x 105 cells per tube were pre incubated 5 minutes with 20 ul of 300 ug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ml of anti-CD278/Biotin at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-PE after which they were washed three times, fixed and analyzed by FACS using a lymphoid gate. Cells stained positive with a mean shift of 1.53 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD278 antibody.

Conjugation

Biotin

Production

Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.

Buffer

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).

Immunogen

Human HPB-MLT cells, recombinant human CD278(ICOS)-muIg

Applications Tested/Suitable for anti-CD278 antibody

Flow Cytometry (FC/FACS), ELISA (EIA)

Product References and Citations for anti-CD278 antibody

1) Beier, K.C., R.A. Kroczek, et al. 2000, Eur J Immunol. 30(12):3707-3717. 2) Riley, J.L., C.H. June, et al. 2001, J. Immunol. 166: 4943-4948. 3) Ling, V., M. Collins, et al. 2000, J. Immunol. 164: 1653-1657. 4) Ling, V., M. Collins, et al. 2001, J. Immunol. 166: 7300-7308.

Other Notes

Small volumes of anti-CD278 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD278 purchase

MBS666514 is an antibody targeting CD278. Also known as anti-human CD278(ICOS)-Biotin. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD278. It may not necessarily be applicable to this product.

Protein Family

Inducible T-cell costimulator

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.