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CD27 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666559

Information: Human CD27 is a lymphocyte specific member of the tumor necrosis factor (TNF) receptor family and is found primarily on peripheral blood T cells and on a subpopulation of B cells and NK cells. The ligand for CD27 is CD70, which is a member of the… More details

$435 / 120 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD27, Monoclonal Antibody
Full Product Name
anti-human CD27-FITC
Product Gene Name
anti-CD27 antibody
Product Synonym Names
Monoclonal anti-human CD27/FITC
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M-T271
Host
Mouse
Species Reactivity
Human, Monkey
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Concentration
Volume: 0.2ml (lot specific)
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Molecular Weight
29,137 Da
OMIM
615122
Performance
Five x 105 ficoll prepared human peripheral blood lymphocytes per tube were washed and pre incubated 5 minutes with 20 ul of 250 ug/ml human IgG (to block non specific binding) after which they were washed and incubated 45 minutes on ice with 80 ul of anti-CD27/FITC at a 1:50 dilution factor(10 ug/ml). Cells were washed three times, fixed and analyzed by FACS. A net 56% subpopulation of the cells stained positive with a mean shift of 1.24 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD27 antibody.
Conjugation
FITC
Production
Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at 0.5 mg/ml with a Fluorescein/IgG molar ratio of 12.8.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen
T cell line (from patient with CLL)
Applications Tested/Suitable for anti-CD27 antibody
Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-CD27 antibody
1:50 (or use 1.6 ul of concentrated stock per 5 x 105-cell test)

Testing Data of anti-CD27 antibody

Mouse Anti-CD27 Monoclonal Antibody – Cat# MBS666559

Product References and Citations for anti-CD27 antibody

Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 350-352. K. Agematsu, et al, (1994) J Immunol 153: 1421-1429. R.Q. Hintzen, et al, (1994) Immunol Today 15: 307-311. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 356-360, 435-437. K. Agematsu, et al, (1995) J Immunol 154: 3627-3635.

Other Notes

Small volumes of anti-CD27 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD27 purchase

MBS666559 is an antibody targeting CD27. Also known as anti-human CD27-FITC. Reactive in Human, Monkey. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD27. It may not necessarily be applicable to this product.

NCBI GI #
4507587
NCBI GeneID
939
NCBI Accession #
NP_001233.1
UniProt Secondary Accession #
B2RDZ0
UniProt Related Accession #
P26842
GenBank Nucleotide #
NM_001242.4
NCBI Official Full Name
CD27 antigen
NCBI Official Synonym Full Names
CD27 molecule
NCBI Official Symbol
CD27
NCBI Official Synonym Symbols
T14; S152; Tp55; TNFRSF7; S152. LPFS2
UniProt Protein Name
CD27 antigen
UniProt Synonym Protein Names
CD27L receptor; T-cell activation antigen CD27; T14; Tumor necrosis factor receptor superfamily member 7; CD_antigen: CD27
Protein Family
CD27 antigen
UniProt Gene Name
CD27
UniProt Synonym Gene Names
TNFRSF7
UniProt Entry Name
CD27_HUMAN
NCBI Protein Information
CD27 antigen; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7

NCBI Summary for CD27

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD27

CD27: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Protein type: Apoptosis; Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: integral to plasma membrane; extracellular region; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; caspase inhibitor activity

Biological Process: release of cytoplasmic sequestered NF-kappaB; cell surface receptor linked signal transduction; response to ethanol; positive regulation of B cell differentiation; positive regulation of JNK cascade; negative regulation of caspase activity; immunoglobulin mediated immune response; negative regulation of apoptosis

Disease: Lymphoproliferative Syndrome 2
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Research Articles on CD27

1. A lower primary CD24(hi) CD27(+) CD19(+) B cells may be an immunologic aspect of new-onset SLE that may be a useful tool to evaluate lupus activity and monitor the response to therapy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD27 antibody

 Reference Product  PubMed Publications
 CD19 antibody  >306 publications with CD27 and CD19
 CD70 antibody  >255 publications with CD27 and CD70
 CD38 antibody  >243 publications with CD27 and CD38
 CCR7 antibody  >209 publications with CD27 and CCR7
 CD5 antibody  >116 publications with CD27 and CD5
 CXCR3 antibody  >47 publications with CD27 and CXCR3
 TRAF2 antibody  >13 publications with CD27 and TRAF2
 SIVA1 antibody  >12 publications with CD27 and SIVA1
 TRAF3 antibody  >8 publications with CD27 and TRAF3
 CD40LG antibody  >1 publications with CD27 and CD40LG

Pathways associated with anti-CD27 antibody

 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram

Diseases associated with anti-CD27 antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >881 publications with CD27 and Immune System Diseases
 Necrosis Antibodies  >420 publications with CD27 and Necrosis
 Lymphoma Antibodies  >222 publications with CD27 and Lymphoma
 Inflammation Antibodies  >165 publications with CD27 and Inflammation
 Nervous System Diseases Antibodies  >100 publications with CD27 and Nervous System Diseases
 Skin Diseases Antibodies  >90 publications with CD27 and Skin Diseases
 Neoplasms, Experimental Antibodies  >79 publications with CD27 and Neoplasms, Experimental
 Liver Diseases Antibodies  >64 publications with CD27 and Liver Diseases
 Carcinoma Antibodies  >59 publications with CD27 and Carcinoma
 Memory Disorders Antibodies  >43 publications with CD27 and Memory Disorders

Organs/Tissues associated with anti-CD27 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2294 publications with CD27 and Blood
 Lymph Node Antibodies  >172 publications with CD27 and Lymph Node
 Spleen Antibodies  >149 publications with CD27 and Spleen
 Lymph Antibodies  >140 publications with CD27 and Lymph
 Lung Antibodies  >85 publications with CD27 and Lung
 Kidney Antibodies  >82 publications with CD27 and Kidney
 Thymus Antibodies  >76 publications with CD27 and Thymus
 Tonsil Antibodies  >61 publications with CD27 and Tonsil
 Intestine Antibodies  >38 publications with CD27 and Intestine
 Heart Antibodies  >36 publications with CD27 and Heart
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