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CD71 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666593

Information: Human CD71 is a type II transmembrane glycoprotein found on activated leukocytes. CD71 binds transferrin and mediates uptake of iron through endocytosis and recycling. Antibody DF1513 recognizes the CD71 molecule of about 95 kd. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD71, Monoclonal Antibody
Full Product Name
anti-human CD71-Purified Preservative Free
Product Gene Name
anti-CD71 antibody
Product Synonym Names
Monoclonal anti-human CD71 (Transferrin receptor)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
DF1513
Host
Mouse
Species Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
Purity/Purification
Purified Preservative Free
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Open under aseptic conditions.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Performance
Five x 105 cultured HPB-MLT cells were washed and pre incubated 5 minutes with 20 mul of 250 mug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 mul of anti-CD71 antibody at 5 mug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.23 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Production
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2mu sterile filtered and vialed under aseptic conditions.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
Immunogen
Human PBL
Applications Tested/Suitable for anti-CD71 antibody
Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), (Not WB)

Testing Data of anti-CD71 antibody

Mouse Anti-CD71 Monoclonal Antibody – Cat# MBS666593

Product References and Citations for anti-CD71 antibody

1)Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 455-460. 2)Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 524-527.

Other Notes

Small volumes of anti-CD71 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD71 purchase

MBS666593 is an antibody targeting CD71. Also known as anti-human CD71-Purified Preservative Free. Reactive in Human. Host: Mouse.
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