Mouse Anti-CD106 Monoclonal Antibody – Cat# MBS666601

CD106 Antibody – Mouse Monoclonal

Name: CD106 Antibody; Monoclonal; Mouse; Human/Pig-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS666601
Price: 530 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666601

Information: Human CD106 is an endothelial adhesion molecule that binds to alpha 4 beta 1 & alpha 4 beta 7 integrins (VLA-4) and promotes adhesion of lymphocytes, monocytes, eosinophils and basophils. CD106 is also found on follicular dendritic cells,… More details

$530 / 120 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD106, Monoclonal Antibody

Full Product Name

anti-human CD106-R-PE

Product Gene Name

anti-CD106 antibody

Product Synonym Names

Monoclonal anti-human CD106(VCAM-1)/R-PE

Clonality

Monoclonal

Isotype

IgG1

Clone Number

1.G11B1

Host

Mouse

Species Reactivity

Human, Pig

Preparation and Storage

Store at 2 - 5 degree C. Do not freeze! Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.

Performance

Cultured human umbilical cord vein endothelial cells were stimulated 4 hours with 10 ng/ml Phorbol 12-Myristate 13-Acetate. Five x 10⁵ cells per tube were harvested, washed and incubated 45 minutes on ice with 80 ul of anti-CD106/R-PE at a 1:50 dilution (10ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.36 log₁₀ fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD106 antibody.

Conjugation

Production

Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.5 mg/ml with an A₅₆₅/A₂₈₀ ratio of 3.53.

Buffer

50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).

Immunogen

Human endothelial cells

Applications Tested/Suitable for anti-CD106 antibody

Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Blocking

Application Notes for anti-CD106 antibody

Dilution: 1:50 (or use 1.6ul of concentrated reagent per 5x10⁵-cell test)

Product References and Citations for anti-CD106 antibody

M.H. Thornhill, D.O. Haskard, (1991) J Immunol 146: 592-598. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1764-1767. M. Nagata, et al, (1995) J Immunol 155: 2194-2202. H.E. Chuluyan, et al, (1995) J Immunol 155: 3135-314.

Other Notes

Small volumes of anti-CD106 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD106 purchase

MBS666601 is an antibody targeting CD106. Also known as anti-human CD106-R-PE. Reactive in Human, Pig. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD106. It may not necessarily be applicable to this product.

Protein Family

Vascular cell adhesion protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.