CD235 Antibody – Mouse Monoclonal

Monoclonal

IgMk

A63-B/C2

Mouse

Human

50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl

Purified Preservative Free

1.0 mg/ml (lot specific)

Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Open under aseptic conditions.
Product should retain activity for at least 12 months after shipping date when stored as recommended.

Ten x 106 human red blood cells were washed and preincubated 5 minutes with 20 ul of 250 ug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD235a (Glycophorin A) antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC; after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.9 log10 fluorescent units when compared to a Mouse IgM negative control at a similar concentration.

Antibody from (low FBS containing) tissue culture supernatant was purified to 95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin) using size exclusion chromatography. Product was 0.2 um filtered and vialed under aseptic conditions.

50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl.

Neuraminidase treated human erythrocytes