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CD63 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666687

Information: Human CD63 is expressed on activated platelets and also on monocytes and macrophages. CD63 contains four hydrophobic transmembrane regions and is a member of the tetraspan family. CD63 appears to be involved with cell adhesion and associates with… More details

$530 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD63, Monoclonal Antibody
Full Product Name
anti-human CD63-Biotin
Product Gene Name
Product Synonym Names
Monoclonal anti-human CD63/Biotin
Clonality
Monoclonal
Isotype
IgG1
Clone Number
AHN16.1/46-4-5
Host
Mouse
Species Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
Store at 2 - 5 degree C. Freeze/thawing not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Molecular Weight
17,271 Da
OMIM
155740
Performance
Five x 105 cultured HPB-MLT human tumor cells were washed and pre incubated 5 minutes with 20 ul of 250 ug/ml human IgG (to block nonspecific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD63/Biotin at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.17 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD63 antibody.
Conjugation
Biotin
Production
Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen
Human eosinophils
Applications Tested/Suitable for anti-CD63 antibody
Flow Cytometry (FC/FACS), Immunocytochemistry (ICC)

Testing Data of anti-CD63 antibody

Mouse Anti-CD63 Monoclonal Antibody – Cat# MBS666687

Product References and Citations for anti-CD63 antibody

F. Berditchevski, et al, (1995) J Biol Chem 270: 17784-17790. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1352-1364.

Other Notes

Small volumes of anti-CD63 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD63 purchase

MBS666687 is an antibody targeting CD63. Also known as anti-human CD63-Biotin. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD63. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
CD63 antigen isoform D
NCBI Official Synonym Full Names
CD63 molecule
NCBI Official Symbol
CD63
NCBI Official Synonym Symbols
MLA1; ME491; LAMP-3; OMA81H; TSPAN30
UniProt Protein Name
CD63 antigen
UniProt Synonym Protein Names
Granulophysin; Lysosomal-associated membrane protein 3; LAMP-3; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; Tetraspanin-30; Tspan-30; CD_antigen: CD63
Protein Family
UniProt Gene Name
CD63
UniProt Synonym Gene Names
MLA1; TSPAN30; LAMP-3; Tspan-30
UniProt Entry Name
CD63_HUMAN
NCBI Protein Information
CD63 antigen; tspan-30; granulophysin; tetraspanin-30; melanoma-associated antigen MLA1; CD63 antigen (melanoma 1 antigen); melanoma-associated antigen ME491; ocular melanoma-associated antigen; lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3

NCBI Summary for CD63

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]
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UniProt Comments for CD63

CD63: This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. Belongs to the tetraspanin (TM4SF) family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q12-q13

Cellular Component: cell surface; lysosomal membrane; integral to plasma membrane; late endosome membrane; plasma membrane; melanosome; endosome membrane; platelet dense granule membrane; intrinsic to plasma membrane

Molecular Function: protein binding

Biological Process: platelet activation; protein transport; cell migration; pigment granule maturation; platelet degranulation; cell-matrix adhesion; positive regulation of receptor internalization; blood coagulation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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