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IgG1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666730

Information: Immunoglobulin expressed by the mouse plasmacytoma MOPC 31C is an IgG1ƒÛ isotype of unknown specificity. It is used as a negative control when working with human cells. More details

$315 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IgG1, Monoclonal Antibody
Full Product Name
Mouse IgG1-Purified Preservative Free
Product Gene Name
Product Synonym Names
Monoclonal Mouse IgG1
Clonality
Monoclonal
Isotype
IgG1k
Clone Number
MOPC31C
Host
Mouse
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
Purity/Purification
Purified Preservative Free
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Open under aseptic conditions.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Cells Tested
Human PBL, CEM, HPB-MLT, Nalm-6, Jurkat, KG-1, U937, Molt-4, and Raji (cells were pre-incubated with human IgG to block nonspecific binding).
Performance
Mouse IgG1 was tested as a negative control in Flow cytometry at 20 ug/ml using Goat anti-Mouse IgG/FITC as a 2 degree reagent. All cells tested failed to stain or display a significant mean shift above a buffer background.
Production
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2u sterile filtered and vialed under aseptic conditions.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
Applications Tested/Suitable for anti-IgG1 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)

Other Notes

Small volumes of anti-IgG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IgG1 purchase

MBS666730 is an antibody targeting IgG1. Also known as Mouse IgG1-Purified Preservative Free. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for IgG1. It may not necessarily be applicable to this product.

Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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