CD154 Human Protein

Name: CD154 Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS666788
Price: 715 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666788

Information: Human CD154 (CD40 Ligand) is a member of the tumor necrosis factor (TNF) family and is expressed on the surface of activated T cells. Interaction of CD154 and CD40 is essential for isotype switching in B cells. Known genetic defects that alter… More details

$715 / 0.025 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD154, Protein

Full Product Name

CD154-muCD8-Biotin

Product Gene Name

CD154 protein

Product Synonym Names

Human CD154 (CD40 Ligand) muCD8/Biotin

Host

CHO cells

Species Reactivity

Human, Pig, Dog, Mouse

Form/Format

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.

Concentration

0.5 mg/ml (lot specific)

Preparation and Storage

Store at 2 - 5 degree C. Freeze/Thawing is not recommended.
Product should retain activity for at least 6 months after shipping date when stored as recommended.

Molecular Structure

A soluble fusion protein consisting of the extracellular domain (213aa) of human CD154 fused to the extracellular domain (167aa) of murine CD8 alpha.

Transfectant Cell Line

CHO

Performance

Five x 105 cultured human Raji cells per tube were washed and incubated 45 minutes on ice with 80 ul of CD154-muCD8/Biotin at a concentration of 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-PE, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.28 log10 fluorescent units when compared to a buffer control at a similar concentration. Binding was blocked when reagent was pre incubated 30 minutes with anti-CD154 antibody at a concentration of 100 ug/ml.

Conjugation

Biotin

Production

Fusion protein from (low FBS containing) tissue culture supernatant of transfectants was purified using affinity and size exclusion chromatography), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate by desalting.

Buffer

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).

Applications Tested/Suitable for CD154 protein

Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)

Product References and Citations for CD154 protein

1) D. Gray, et al, (1994) Seminars in Immunol 6: 303-310. 2) F. Pietravalle, et al, (1996) J Biol Chemistry 271: 5965-5967. 3) R.J. Noelle, (1996) Immunity 4: 415-419. 4) A. Desai-Mehta, et al, (1996) J Clin Invest 97: 2063-2073. 5) I.S. Grewal and R.A. Flavell, (1996) Immunol Today 17: 410-414. 6) F. Mach, et al, (1997) Proc Natl Acad Sci USA 94:1931-1936.

Other Notes

Small volumes of CD154 protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD154 purchase

MBS666788 is a protein targeting CD154. Also known as CD154-muCD8-Biotin. Reactive in Human, Pig, Dog, Mouse. Host: CHO cells.

Additional Details

NCBI/Uniprot data below describe general gene information for CD154. It may not necessarily be applicable to this product.

Protein Family

CD40 ligand

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.