CD278 Human Protein

CHO cells

Human

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl

Purified Preservative Free

0.5 mg/ml (lot specific)

Store at 2 - 5 degree C. Open under aseptic conditions. Freeze/Thawing is not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.

A soluble molecule consisting of the extracellular (121aa) domain of mature human ICOS fused to the murine IgG2a Fc (232 aa). Predicted monomeric weight is 42.8 kd (amino acid composition only).

CHO

ICOS-muIg fusion protein binds to Raji cells in FACS. Five x 105 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 ul of ICOS-muIg at 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.42 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Binding was blocked when reagent was pre incubated with 100 ug/ml of recombinant GL50(ICOSL)-muIg. ICOS-muIg fusion protein was reactive in EIA using a Goat-anti-mouse-Ig antibody capture, followed by detection using GL50-muIg/Biotin and Streptavidin/HRP. Amino acid sequence of fusion protein was confirmed by n-terminal analysis (EINGS).

Fusion protein from (low FBS containing) tissue culture supernatant of transfectants was purified using size exclusion chromatography. Product was 0.2u sterile-filtered and vialed under aseptic conditions.

50 mM Sodium Phosphate pH 7.6, 100mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).