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CD274 Human Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666818

Information: CD274 (B7-H1, PD-L1, Programmed Death Ligand) is a member of the B7 family and is expressed on a variety of tissues including lymphoid cells. It plays an important role in regulation of T cell activation, and is involved in progression of cancer,… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD274, Protein
Full Product Name
CD274 (B7-H1)-muIg-Biotin
Product Gene Name
CD274 protein
Product Synonym Names
Human CD274(B7-H1)-muIg/Biotin Fusion Protein
Sequence
MuCD8 alpha signal peptide residual amino acids + linker:(1) kpqapelrgs as

CD274 mature EC: (224aa): (13)ftvtvp kdlyvveygs nmtieckfpv ekqldlaali vywemedkni iqfvhgeedl kvqhssyrqr arllkdqlsl gnaalqitdv klqdagvyrc misyggadyk ritvkvnapy nkinqrilvv dvtseheltc qaegypkaev iwtssdhqvl sgkttttnsk reeklfnvts tlrintttne ifyctfrrld peenhtaelv ipelplahpp nerthtr

Linke r + Murine IgG2a Hinge + Fc (235aa):(237)gte prgptikpcp pckcpapnll ggpsvfifpp kikdvlmisl spivtcvvvd vseddpdvqi swfvnnvevh taqtqthred ynstlrvvsa lpiqhqdwms gkefkckvnn kdlpapiert iskpgsvrap qvyvlpppee emtkkqvtlt cmvtdfmped iyvewtnngk telnykntep vldsdgsyfm ysklrvekkn wvernsyscs vvheglhnhh ttksfsrtpg (471)
Host
CHO cells
Species Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Concentration
0.5 mg/ml (lot specific)
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended
Product should retain activity for at least 6 months after shipping date when stored as recommended.
OMIM
605402
Molecular Structure
A soluble fusion protein consisting of the murine CD8 alpha leader sequence, the mature extracellular (224aa) domain of human CD274 fused to murine IgG2a Fc + hinge (233aa).
Transfectant Cell Line
CHO
Performance
Purity of recombinant protein was >90% by SDS-PAGE. N-terminal sequence was as predicted: KPQAP. Biotinylated recombinant protein was active in EIA, binding to anti-CD274 mAbs ANC6H1, clone M1H1, and recombinant CD279(PD1), using SA/HRP for detection.

Predicted monomeric (non glycosylated) molecular weight: 54.4kd. The molecule is dimeric and runs at about 120 kd in SDS-PAGE under native conditions.
Conjugation
Biotin
Production
Human CD274-muIg fusion protein was Protein A purified from (low FBS containing) tissue culture supernatant of CHO transfectants, and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate by desalting column.
Buffer
50mM Sodium Phosphate pH7.5, 100mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Molecular Weight
Predicted monomeric (non glycosylated) molecular weight: 54.4kd.
The molecule is dimeric and runs at about 120 kd in SDS-PAGE under native conditions.
Applications Tested/Suitable for CD274 protein
ELISA (EIA)

Product References and Citations for CD274 protein

1)Cancer Research (April 2006) 66:3381. 2)J Molecular Medicine (Feb 2004) 81(5):281. 3)Int J Hematol (Nov 2003) 78(4):321.

Other Notes

Small volumes of CD274 protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD274 purchase

MBS666818 is a protein targeting CD274. Also known as CD274 (B7-H1)-muIg-Biotin. Reactive in Human. Host: CHO cells.

Additional Details

NCBI/Uniprot data below describe general gene information for CD274. It may not necessarily be applicable to this product.

NCBI GI #
112363645
NCBI GeneID
29126
NCBI Accession #
ABI16084.1
UniProt Secondary Accession #
Q14CJ2; Q2V8D5; Q66RK1; Q6WEX4; Q9NUZ5; B2RBA2; B4DU27
UniProt Related Accession #
Q9NZQ7
NCBI Official Full Name
CD274, partial
NCBI Official Synonym Full Names
CD274 molecule
NCBI Official Symbol
CD274
NCBI Official Synonym Symbols
B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
UniProt Protein Name
Programmed cell death 1 ligand 1
UniProt Synonym Protein Names
B7 homolog 1; B7-H1; CD_antigen: CD274
Protein Family
Programmed cell death
UniProt Gene Name
CD274
UniProt Synonym Gene Names
B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
UniProt Entry Name
PD1L1_HUMAN
NCBI Protein Information
programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1

UniProt Comments for CD274

CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: integral to membrane; plasma membrane; endomembrane system; external side of plasma membrane

Molecular Function: protein binding

Biological Process: regulation of activated T cell proliferation; cell surface receptor linked signal transduction; negative regulation of interleukin-10 production; negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; T cell costimulation; positive regulation of T cell proliferation; immune response; signal transduction
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Research Articles on CD274

1. PD-L1 expression on myeloid precursor cells is increased quantitatively and qualitatively in AML patients after gamma-interferon treatment initiation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CD274 protein

 Reference Product  PubMed Publications
 CD80 protein  >390 publications with CD274 and CD80
 PDCD1 protein  >322 publications with CD274 and PDCD1
 STAT1 protein  >11 publications with CD274 and STAT1
 PTEN protein  >8 publications with CD274 and PTEN

Pathways associated with CD274 protein

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 PD-1 Signaling Pathway antibodies  PD-1 Signaling Pathway Diagram

Diseases associated with CD274 protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >106 publications with CD274 and Inflammation
 Liver Diseases Antibodies  >84 publications with CD274 and Liver Diseases
 Necrosis Antibodies  >73 publications with CD274 and Necrosis
 Nervous System Diseases Antibodies  >69 publications with CD274 and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >65 publications with CD274 and Neoplasms, Experimental
 Adenocarcinoma Antibodies  >59 publications with CD274 and Adenocarcinoma
 Lung Diseases Antibodies  >44 publications with CD274 and Lung Diseases
 Kidney Diseases Antibodies  >43 publications with CD274 and Kidney Diseases
 Lung Neoplasms Antibodies  >40 publications with CD274 and Lung Neoplasms
 Recurrence Antibodies  >26 publications with CD274 and Recurrence

Organs/Tissues associated with CD274 protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >755 publications with CD274 and Blood
 Lymph Node Antibodies  >78 publications with CD274 and Lymph Node
 Lung Antibodies  >70 publications with CD274 and Lung
 Bone Antibodies  >58 publications with CD274 and Bone
 Skin Antibodies  >45 publications with CD274 and Skin
 Brain Antibodies  >31 publications with CD274 and Brain
 Pancreas Antibodies  >28 publications with CD274 and Pancreas
 Placenta Antibodies  >19 publications with CD274 and Placenta
 Stomach Antibodies  >13 publications with CD274 and Stomach
 Mouth Antibodies  >9 publications with CD274 and Mouth
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