CD54 Human Protein

Name: CD54 Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS666833
Price: 715 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666833

Information: Human CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). CD54 expression on resting peripheral blood leukocytes is weak but is upregulated on activated T and B lymphocytes and on… More details

$715 / 0.025 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD54, Protein

Full Product Name

CD54-muIg-Biotin

Product Gene Name

CD54 protein

Product Synonym Names

Human CD54(ICAM-1) muIg/Biotin

Host

CHO cells

Species Reactivity

Human

Form/Format

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.

Concentration

0.5 mg/ml (lot specific)

Preparation and Storage

Store at 2 - 5 degree C. Freeze/Thawing is not recommended
Product should retain activity for at least 12 months after shipping date when stored as recommended.

Molecular Structure

A soluble dimeric fusion protein consisting of the extracellular (451aa) domain of human CD54 (including signal peptide) fused to murine IgG2a Fc (232aa). Predicted monomeric molecular weight of mature construct 76.1kd (amino acid composition only).

Transfectant Cell Line

CHO

Performance

Pre incubation with a 10-fold excess of unconjugated CD54-muIg (514-020) blocks binding of anti-CD54(Domain1)/R-PE, and anti-CD54(Domain 2)/R-PE to Raji cells in Flow cytometry. CD54-muIg/Biotin was detectable in EIA at 0.5 ng/ml using anti-CD54(D1) coated plates followed by Streptavidin/HRP and TMB/H2 O2 substrate chromagen for detection.

Conjugation

Biotin

Production

Fusion protein from (low FBS containing) tissue culture supernatant of transfectants was purified using affinity and size exclusion chromatography, and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column

Buffer

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).

Applications Tested/Suitable for CD54 protein

ELISA (EIA)

Product References and Citations for CD54 protein

A.R. Berendt, et al, (1992) Cell 68: 71-81. I. Dransfield, et al, (1992) J Cell Biol 116: 1527-1535. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1548-1550. P.L. Reilly, et al, (1995) J Immunol 155: 529-532.

Other Notes

Small volumes of CD54 protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD54 purchase

MBS666833 is a protein targeting CD54. Also known as CD54-muIg-Biotin. Reactive in Human. Host: CHO cells.

Additional Details

NCBI/Uniprot data below describe general gene information for CD54. It may not necessarily be applicable to this product.

Protein Family

Intercellular adhesion molecule

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.