CD137L Human Protein

CHO cells

Human

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate

Purified (with Antibiotics)

0.5 mg/ml (lot specific)

Store at 2 - 5 degree C. Freeze/Thawing is not recommended.
Product should retain activity for at least 6 months after shipping date when stored as recommended.

A soluble fusion protein consisting of the extracellular domain (184aa) of human CD137 Ligand fused to the extracellular domain (167aa) of murine CD8 alpha.

CHO

Cultured human CEM cells were stimulated 1 day in the presence of PMA (10 ng/ml) and ionomycin (1 muM). Five x 105 cells were washed and incubated 45 minutes on ice with 80 mul of CD137L-muCD8 at 10 mug/ml. Cells were washed twice and incubated with 2 degree reagent anti-mouse CD8alpha/R-PE, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.02 log10 fluorescent units when compared to a buffer control at a similar concentration. Binding was blocked when reagent was pre incubated 30 minutes with 50 mug/ml CD137-huIg.

Fusion protein from (low FBS containing) tissue culture supernatant of transfectants was purified using affinity chromatography.

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).

CD137L-muCD8