CD152 Human Protein

Name: CD152 Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS666880
Price: 1090 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666880

Information: Immune response mediated by T cells can be characterized to functionally proceed as follows: antigen recognition by the T cell receptor, activation through costimulation, effector activities to eliminate antigen and finally down regulation. Human… More details

$1090 / 50 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD152, Protein

Full Product Name

CD152(CTLA-4)-muIg-R-PE

Product Gene Name

CD152 protein

Product Synonym Names

Human CD152(CTLA-4)-muIg/R-PE Fusion Protein (also binds to mouse CD80/CD86)

Host

BHK cells

Species Reactivity

Human, Primate, Mouse, Pig, Cow, Cat

Form/Format

50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA.

Concentration

Volume: 200 ul (lot specific)

Preparation and Storage

Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light
Product should retain activity for at least 12 months after shipping date when stored as recommended.

Molecular Structure

A soluble 110 kd dimeric fusion protein consisting of the extracellular (125aa) domain of human CD152 (CTLA-4) fused to murine IgG2a Fc

Transfectant Cell Line

BHK

Performance

Five x 105 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 mul of CD152-muIg/R-PE at a dilution factor of 1:20 (10 mug/ml). Cells were washed three times, fixed and analyzed using by FACS. The cells stained positive with a mean shift of 1.97 log10 fluorescent units when compared to a Mouse IgG2a/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 mul of a mixture of 0.5 mg/ml each anti-CD80, and anti-CD86 antibodies.

Conjugation

Production

Human CD152-muIg fusion protein from tissue culture supernatant of BHK transfectants was Protein A purified to >95% by SDS-PAGE (<1% bovine immunoglobulin), and conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated fusion protein was removed using size exclusion chromatography. CD152-muIg/R-PE conjugate is at 0.2 mg/ml with an A565/A280 ratio of 3.97.

Buffer

50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative)

Applications Tested/Suitable for CD152 protein

Flow Cytometry (FC/FACS), ELISA (EIA)

Application Notes for CD152 protein

1:20 (or use 4 mul of concentrated stock per 5 x 105-cell test)

Product References and Citations for CD152 protein

T. Lindsten, et al, (1993) J Immunol 151: 3489-3499. T.L. Walunas, et al, (1994) Immunity 1: 405-413. N.J. Karandikar, et al, (1996) J Exp Med 184: 783-788. A.H. Cross, et al, (1995) J Clin Invest 95: 2783-2789. P.A. Morton, et al, (1996) J Immunol 156: 1047-1054. Martin K. Oaks and Karen M. Hallett, (2000) J Immunol 164: 5015-5018. S.J. Fass, et al, (2000) J Immunol 164: 6340-6348.

Other Notes

Small volumes of CD152 protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD152 purchase

MBS666880 is a protein targeting CD152. Also known as CD152(CTLA-4)-muIg-R-PE. Reactive in Human, Primate, Mouse, Pig, Cow, Cat. Host: BHK cells.

Additional Details

NCBI/Uniprot data below describe general gene information for CD152. It may not necessarily be applicable to this product.

Protein Family

Cytotoxic T-lymphocyte protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.