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CD66b Antibody – Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666893

Human CD66b (CEACAM8, CGMb, NCA-95, CD67) is a 95kD member of the Carcinoembryonic Antigen (CEA) group. It is expressed on granulocytes and functions as an adhesion molecule. Similar to the CD66 a, c, and d isoforms, engagement of CD66b on neutrophils causes… More details

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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD66b (CEACAM8), Monoclonal Antibody
Full Product Name
Anti-Human CD66b (CEACAM8)
Product Gene Name
Product Synonym Gene Name
CEACAM8; CGMb; NCA-95; CD67
Product Synonym Names
anti-CD66b (ANC3C5)FITC Conjugate; Monoclonal anti-human CD66b (CEACAM8)/FITC*
Clonality
Monoclonal
Isotype
IgG1K
Clone Number
ANC3C5
Form/Format
FITC
Purity/Purification
Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at approximately 0.5 mg/ml with a Fluorescein/IgG molar rat
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Molecular Weight
38,154 Da
OMIM
615747
Immunogen
Recombinant soluble human CD66b
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Performance
Five x 105 Polymorphprep prepared human Granulocytes(PMN) per tube were pelleted and pre incubated 5 minutes with 20ul of human IgG at 300ug/ml (to reduce non specific binding) after which they were washed and incubated 45 minutes on ice with 80 ul of anti-CD66b(ANC3C5) /FITC at a 1:50 dilution (10 ug/ml). They were then washed three times, fixed and analyzed by FACS. A 53% sub population of the cells stained positive with a mean shift of 1.22 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD66b(ANC3C5) antibody.

Product Categories/Family for anti-CD66b antibody

Applications Tested/Suitable for anti-CD66b antibody
Flow Cytometry (FC/FACS), ELISA (EIA)

Flow Cytometry (FC/FACS) of anti-CD66b antibody

Anti-CD66b Monoclonal Antibody – Cat# MBS666893

Product References and Citations for anti-CD66b antibody

1) W.N. Khan, S. Hammarstrom, et al. (1992) Genomics 14(2):384-90. 2) K.M. Skubitz, A.P. Skubitz, et al. (1996) J Leuk Biol 60(1): 106-117. 3) A.K. Schroder, L Rink, et al. (2006) Human Immunol 67(9): 676-682.

Other Notes

Small volumes of anti-CD66b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD66b purchase

MBS666893 is an antibody targeting CD66b. Also known as Anti-Human CD66b (CEACAM8).

Additional Details

NCBI/Uniprot data below describe general gene information for CD66b. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
carcinoembryonic antigen-related cell adhesion molecule 8
NCBI Official Synonym Full Names
carcinoembryonic antigen related cell adhesion molecule 8
NCBI Official Symbol
CEACAM8
NCBI Official Synonym Symbols
CD67; CGM6; CD66b; NCA-95
UniProt Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 8
UniProt Synonym Protein Names
CD67 antigen; Carcinoembryonic antigen CGM6; Non-specific cross-reacting antigen NCA-95; CD_antigen: CD66b
UniProt Gene Name
CEACAM8
UniProt Synonym Gene Names
CGM6
UniProt Entry Name
CEAM8_HUMAN
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 8

UniProt Comments for CD66b

CEACAM8: Belongs to the immunoglobulin superfamily. CEA family.

Protein type: Membrane protein, integral; Membrane protein, GPI anchor; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: protein binding

Biological Process: immune response; leukocyte migration
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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