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CD11b Antibody – Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666899

Human CD11b (alpha M integrin) complexes with CD18 (beta 2 integrin) to form the complement receptor type 3 (CR3) heterodimer which binds to ICAM-1, fibrinogen, C3b, and coagulation factor X. CD11b expression is restricted to leukocytes, mainly to myeloid and… More details

$530 / 120 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD11b, Monoclonal Antibody
Full Product Name
Anti-Human CD11b
Product Gene Name
Product Synonym Gene Name
CR3; Mac-1
Product Synonym Names
anti-CD11bAllophycocyanin; Monoclonal anti-human CD11b/APC*; Integrin alpha M
Clonality
Monoclonal
Isotype
IgG1
Clone Number
ICR44
Form/Format
Allophycocyanin
Purity/Purification
Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to 95% Immunoglobulin by SDS-PAGE, and conjugated to Allophycocyanin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography.
Preparation and Storage
Store at 2 - 5 degree C. Do not freeze! Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Molecular Weight
29,532 Da
Immunogen
Human Monocytes
Buffer
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Performance
Five x 105 ficoll prepared human PBMCwere washed and pre incubated ~10 minutes with 20 ul human IgG at 250 ug/ml (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD11b/APC at a 1:50dilution factor (10ug/ml). Cells were washed three times, fixed and analyzed by FACS. A 10.7% sub population of the cells stained positive with a mean shift of 1.7 log 10 fluorescent units when compared to a Mouse IgG1/APC negative control. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD11b antibody.

Product Categories/Family for anti-CD11b antibody

Applications Tested/Suitable for anti-CD11b antibody
Flow Cytometry (FC/FACS)

Flow Cytometry (FC/FACS) of anti-CD11b antibody

Anti-CD11b Monoclonal Antibody – Cat# MBS666899

Product References and Citations for anti-CD11b antibody

1. V. Mathotra, et al, (1986) Eur J Immunol 16: 1117-1123. 2. B.L. Myones, et al, (1988) J Clin Invest 82: 640-651. 3. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 555-558, 560-562. 4. H.R. Petty & R.F. Todd, (1993) J Leuk Biol 54: 492-494. 5. Leukocyte Typig V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995)p. 1588-1590. 6. Yoshino N, Honda M, et al. (2000) Exp Anim 49(2):97-110.

Other Notes

Small volumes of anti-CD11b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD11b purchase

MBS666899 is an antibody targeting CD11b. Also known as Anti-Human CD11b.

Additional Details

NCBI/Uniprot data below describe general gene information for CD11b. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
CD11B, partial
UniProt Protein Name
CD11B
UniProt Synonym Protein Names
CD11B
Protein Family
UniProt Gene Name
CD11B
UniProt Entry Name
A0A0S7HMM4_9TELE

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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