CD326 Antibody – Monoclonal

Monoclonal

IgG1K

ANC8D4

FITC

Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at approximately 0.5 mg/ml with a Fluorescein/IgG molar rat

Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.

Recombinant soluble human CD326

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).

Cultured human SW480 cells were harvested by EDTA. Five x 105 cells per tube were pre incubated with 20 ul of 300 ug/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of product at a 1:50 dilution (10 ug/ml). They were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.30 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD326 antibody.