CD152 Recombinant Protein – Cat# MBS666917

CD152 (CTLA-4) (CD152) Recombinant Protein

Name: CD152 Recombinant Protein; CD152 (CTLA-4); Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS666917
Price: 1090 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666917

Human CD152 (CTLA-4) is a cell surface glycoprotein expressed at low levels on activated T cells (1). CD152 is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell… More details

$1090 / 50 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD152 (CTLA-4), Recombinant Protein

Full Product Name

CD152 (CTLA-4)-muIg

Product Gene Name

CD152 recombinant protein

Product Synonym Gene Name

CTLA-4

Product Synonym Names

CD152(CTLA-4)-muIgAllophycocyanin; Human CD152(CTLA-4)-muIg/APC Fusion Protein*

Form/Format

Allophycocyanin

Purity/Purification

Human CD152-muIg fusion protein from tissue culture supernatant of BHK transfectants was Protein A purified to >95% by SDS-PAGE (<1% bovine immunoglobulin), and conjugated to Allophycocyanin through a sulfo-ester linkage. Unconjugated fusion protein was r

Preparation and Storage

Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.

Buffer

50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative)

Performance

Five x 105 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 ul of CD152-muIg/APC at a dilution factor of 1:20 (10 ug/ml). Cells were washed three times, fixed and analyzed using by FACS. The cells stained positive with a mean shift of 2.2 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated with 20 ul of 0.5 mg/ml unconjugated CD152-muIg.

Product Categories/Family for CD152 recombinant protein

Recombinant Proteins

Applications Tested/Suitable for CD152 recombinant protein

Flow Cytometry (FC/FACS), ELISA (EIA)

Product References and Citations for CD152 recombinant protein

1. T. Lindsten, et al, (1993) J Immunol 151: 3489-3499. 2. T.L. Walunas, et al, (1994) Immunity 1: 405-413. 3. N.J. Karandikar, et al, (1996) J Exp Med 184: 783-788. 4. A.H. Cross, et al, (1995) J Clin Invest 95: 2783-2789. 5. P.A. Morton, et al, (1996) J Immunol 156: 1047-1054. 6). Takamatsu H, RME Parkhouse, et al. (1999) Immunology 97(2):211-213. 7). Le Leduec JB, B Dubois, et al. (2016) Vaccine 34(7): 914-22. PMID: 26768129

Other Notes

Small volumes of CD152 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD152 purchase

MBS666917 is a recombinant protein targeting CD152. Also known as CD152 (CTLA-4)-muIg.